This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yoneda, Y Articles by Maruo, B Search for Related Content PubMed PubMed Citation Articles by Yoneda, Y Articles by Maruo, B

 Previous Article  |  Next Article 

J Bacteriol. 1975 October; 124(1): 48-54

Mutation of Bacillus subtilis causing hyperproduction of alpha-amylase and protease, and its synergistic effect.

ABSTRACT

Mutants that had a genetic lesion increasing the production of alpha-amylase and protease simultaneously were isolated from a transformable strain of Bacillus subtilis Marburg by N-methyl-N'-nitro-N-nitrosoguanidine treatment. These mutants produced two to three times more alpha-amylase and five to 16 times more protease than their parent and were tentatively referred to as AP mutants. As this mutation seems to have occurred at a single gene of the bacterial chromosome and was not located near the alpha-amylase structural gene, the gene was designated as "pap." When pap- and amyR2 (an alpha amylase regulator gene) or pap- and ProH coexisted in the same cell, synergistic effects of the two genetic characters were observed on the alpha-amylase and protease production, respectively. Upon introduction of the pap mutation, the following phenotypic changes were observed in addition to changes in alpha-amylase and protease productivity. (i) Mutants lost the character of competence for the transformation. (ii) When cells were cultured at 30 C for 30 h, mutant cells became filament owing to the formation of chains of cells. (iii) Autolysis of cells was decreased in the mutants. When pap- was transferred to the wild strain by deoxyribonucleic acid-mediated transformation, the transformants showed all these phenotypic alterations simultaneously.

This article has been cited by other articles:

• Kurosawa, K., Hosaka, T., Tamehiro, N., Inaoka, T., Ochi, K. (2006). Improvement of {alpha}-Amylase Production by Modulation of Ribosomal Component Protein S12 in Bacillus subtilis 168. Appl. Environ. Microbiol. 72: 71-77 [Abstract] [Full Text]  
• Buist, G., Venema, G., Kok, J. (1998). Autolysis of Lactococcus lactis Is Influenced by Proteolysis. J. Bacteriol. 180: 5947-5953 [Abstract] [Full Text]