This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cabib, E Articles by Bowers, B Search for Related Content PubMed PubMed Citation Articles by Cabib, E Articles by Bowers, B

 Previous Article  |  Next Article 

J Bacteriol. 1975 December; 124(3): 1586-1593

Timing and function of chitin synthesis in yeast.

E Cabib and B Bowers

ABSTRACT

A temperature-sensitive mutant of Saccharomyces cerevisiae, L-2-42, is blocked at 37 C at a stage of the cell cycle prior to septum formation. When single cells of the mutant are allowed to bud at 37 C in a medium containing tritiated glucose, a large incorporation of radioactivity into chitin takes place. Thus, the synthesis of chitin, the major component of the primary septum, is initiated in a phase of the cell cycle which precedes septum closure. This early period of chitin synthesis is not required for emergence and growth of buds because, in the wild type, budding takes place normally in the presence of concentrations of polyoxin D that effectively and specifically prevent chitin formation. However, at a later time a majority of these cells lyse, presumably because of the inability to form a septum. Polyoxin D also prevents the appearance of enhanced fluorescence at the junction between mother cell and bud, as observed in the presence of a brightener. Therefore, the fluorescence is due to chitin and its presence at the base of very early buds indicates that chitin synthesis begins at or shortly after bud emergence. A scheme for chitin synthesis and primary septum formation which embodies these and other results is presented.

---

J Bacteriol. 1975 December; 124(3): 1586-1593

This article has been cited by other articles:

• Lachke, S. A., Joly, S., Daniels, K., Soll, D. R. (2002). Phenotypic switching and filamentation in Candida glabrata. Microbiology 148: 2661-2674 [Abstract] [Full Text]  
• Park, M. H., Joe, Y. A., Kang, K. R. (1998). Deoxyhypusine Synthase Activity Is Essential for Cell Viability in the Yeast Saccharomyces cerevisiae. J. Biol. Chem. 273: 1677-1683 [Abstract] [Full Text]  
• Yu, G, Swiston, J, Young, D (1994). Comparison of human CAP and CAP2, homologs of the yeast adenylyl cyclase-associated proteins. J. Cell Sci. 107: 1671-1678 [Abstract]  
• Duran, A, Cabib, E, Bowers, B (1979). Chitin synthetase distribution on the yeast plasma membrane. Science 203: 363-365 [Abstract]  
• Sloat, B., Pringle, JR (1978). A mutant of yeast defective in cellular morphogenesis. Science 200: 1171-1173 [Abstract]