This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Birken, S Articles by Pisano, M A Search for Related Content PubMed PubMed Citation Articles by Birken, S Articles by Pisano, M A

 Previous Article  |  Next Article 

J Bacteriol. 1976 January; 125(1): 225-232

Purification and properties of polyol dehydrogenase from Cephalosporium chrysogenus.

ABSTRACT

A polyol dehydrogenase of broad specificity was purified 178-fold from extracts of the filamentous fungus Cephalosporium chrysogenum. The enzyme was found to act as an oxido-reductase in two substrate-coenzyme systems: D-sorbitol (or xylitol)-nicotinamide-adenine dinucleotide (NAD) and D-mannitol-nicotinamide adenine dinucleotide phosphate (NADP). The dehydrogenase was composed of five isozymes, which, as a mixture, exhibited these properties: Km to D-sorbitol and D-mannitol, 7.15 to 10(-2) M; PH optimum, 9 to 10; molecular weight, 300,000 subunit weight, 29,000; PI, 5.8 to 7.5. The NADP-linked activity was labile to treatment with heat or ethylenediaminetetraacetic acid. Mixed substrate assays support the hypothesis that both NAD-, and NADP-linked activities are associated with isozymes of a single dehydrogenase.

This article has been cited by other articles:

• Lee, J.-K., Koo, B.-S., Kim, S.-Y., Hyun, H.-H. (2003). Purification and Characterization of a Novel Mannitol Dehydrogenase from a Newly Isolated Strain of Candida magnoliae. Appl. Environ. Microbiol. 69: 4438-4447 [Abstract] [Full Text]  
• Lee, J.-K., Kim, S.-Y., Ryu, Y.-W., Seo, J.-H., Kim, J.-H. (2003). Purification and Characterization of a Novel Erythrose Reductase from Candida magnoliae. Appl. Environ. Microbiol. 69: 3710-3718 [Abstract] [Full Text]  
• Lee, J.-K., Jung, H.-M., Kim, S.-Y. (2003). 1,8-Dihydroxynaphthalene (DHN)-Melanin Biosynthesis Inhibitors Increase Erythritol Production in Torula corallina, and DHN-Melanin Inhibits Erythrose Reductase. Appl. Environ. Microbiol. 69: 3427-3434 [Abstract] [Full Text]  
• Lee, J.-K., Koo, B.-S., Kim, S.-Y. (2002). Fumarate-Mediated Inhibition of Erythrose Reductase, a Key Enzyme for Erythritol Production by Torula corallina. Appl. Environ. Microbiol. 68: 4534-4538 [Abstract] [Full Text]