This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Parkinson, J S Search for Related Content PubMed PubMed Citation Articles by Parkinson, J S

 Previous Article  |  Next Article 

J Bacteriol. 1976 May; 126(2): 758-770

cheA, cheB, and cheC genes of Escherichia coli and their role in chemotaxis.

ABSTRACT

Motile but generally nonchemotatic (che) mutants of Escherichia coli were isolated by a simple screening method. A total of 172 independent mutants were examined, and four genes were defined on the basis of mapping and complemenvestigated by determining their null phenotypes with nonsense or bacteriophage Mu-induced mutations. The cheA and cheB products were essential in producing changes of swimming direction and flagellar rotation. The checC product appeared to be an essential component of the flagellum; however, specific mutational alterations of this component allowed flagellar assembly but prevented directional changes in swimming. Since some cheB mutants changed directions incessantly, this gene product may also serve to control the direction of flagellar rotation in response to chemoreceptor signals. Thus most or all of the common elements in the signalling process were involved in the generation and regulation of changes in the direction of flagellar rotation.

This article has been cited by other articles:

• Kim, T.-J., Young, B. M., Young, G. M. (2008). Effect of Flagellar Mutations on Yersinia enterocolitica Biofilm Formation. Appl. Environ. Microbiol. 74: 5466-5474 [Abstract] [Full Text]  
• Zhao, J., Parkinson, J. S. (2006). Cysteine-Scanning Analysis of the Chemoreceptor-Coupling Domain of the Escherichia coli Chemotaxis Signaling Kinase CheA.. J. Bacteriol. 188: 4321-4330 [Abstract] [Full Text]  
• Zhao, J., Parkinson, J. S. (2006). Mutational Analysis of the Chemoreceptor-Coupling Domain of the Escherichia coli Chemotaxis Signaling Kinase CheA. J. Bacteriol. 188: 3299-3307 [Abstract] [Full Text]  
• del Carmen Buron-Barral, M., Gosink, K. K., Parkinson, J. S. (2006). Loss- and Gain-of-Function Mutations in the F1-HAMP Region of the Escherichia coli Aerotaxis Transducer Aer.. J. Bacteriol. 188: 3477-3486 [Abstract] [Full Text]  
• Gosink, K. K., del Carmen Buron-Barral, M., Parkinson, J. S. (2006). Signaling Interactions between the Aerotaxis Transducer Aer and Heterologous Chemoreceptors in Escherichia coli.. J. Bacteriol. 188: 3487-3493 [Abstract] [Full Text]  
• Yao, J., Allen, C. (2006). Chemotaxis Is Required for Virulence and Competitive Fitness of the Bacterial Wilt Pathogen Ralstonia solanacearum.. J. Bacteriol. 188: 3697-3708 [Abstract] [Full Text]  
• Studdert, C. A., Parkinson, J. S. (2005). Insights into the organization and dynamics of bacterial chemoreceptor clusters through in vivo crosslinking studies. Proc. Natl. Acad. Sci. USA 102: 15623-15628 [Abstract] [Full Text]  
• Bibikov, S. I., Miller, A. C., Gosink, K. K., Parkinson, J. S. (2004). Methylation-Independent Aerotaxis Mediated by the Escherichia coli Aer Protein. J. Bacteriol. 186: 3730-3737 [Abstract] [Full Text]  
• Jahreis, K., Morrison, T. B., Garzon, A., Parkinson, J. S. (2004). Chemotactic Signaling by an Escherichia coli CheA Mutant That Lacks the Binding Domain for Phosphoacceptor Partners. J. Bacteriol. 186: 2664-2672 [Abstract] [Full Text]  
• Studdert, C. A., Parkinson, J. S. (2004). Crosslinking snapshots of bacterial chemoreceptor squads. Proc. Natl. Acad. Sci. USA 101: 2117-2122 [Abstract] [Full Text]  
• Lux, R., Sim, J.-H., Tsai, J. P., Shi, W. (2002). Construction and Characterization of a cheA Mutant of Treponema denticola. J. Bacteriol. 184: 3130-3134 [Abstract] [Full Text]  
• Ames, P., Studdert, C. A., Reiser, R. H., Parkinson, J. S. (2002). From the Cover: Collaborative signaling by mixed chemoreceptor teams in Escherichia coli. Proc. Natl. Acad. Sci. USA 99: 7060-7065 [Abstract] [Full Text]  
• Golden, N. J., Acheson, D. W. K. (2002). Identification of Motility and Autoagglutination Campylobacter jejuni Mutants by Random Transposon Mutagenesis. Infect. Immun. 70: 1761-1771 [Abstract] [Full Text]  
• Lybarger, S. R., Maddock, J. R. (1999). Clustering of the Chemoreceptor Complex in Escherichia coli Is Independent of the Methyltransferase CheR and the Methylesterase CheB. J. Bacteriol. 181: 5527-5529 [Abstract] [Full Text]  
• Elowitz, M. B., Surette, M. G., Wolf, P.-E., Stock, J. B., Leibler, S. (1999). Protein Mobility in the Cytoplasm of Escherichia coli. J. Bacteriol. 181: 197-203 [Abstract] [Full Text]  
• Shukla, D., Zhu, X. Y., Matsumura, P. (1998). Flagellar Motor-switch Binding Face of CheY and the Biochemical Basis of Suppression by CheY Mutants That Compensate for Motor-switch Defects in Escherichia coli. J. Biol. Chem. 273: 23993-23999 [Abstract] [Full Text]  
• Burkart, M., Toguchi, A., Harshey, R. M. (1998). The chemotaxis system, but not chemotaxis, is essential for swarming motility in Escherichia coli. Proc. Natl. Acad. Sci. USA 95: 2568-2573 [Abstract] [Full Text]  
• Zhu, X., Volz, K., Matsumura, P. (1997). The CheZ-binding Surface of CheY Overlaps the CheA- and FliM-binding Surfaces. J. Biol. Chem. 272: 23758-23764 [Abstract] [Full Text]  
• Muskavitch, M., Kort, E., Springer, M., Goy, M., Adler, J (1978). Attraction by repellents: an error in sensory information processing by bacterial mutants. Science 201: 63-65 [Abstract]  
• Bibikov, S. I., Barnes, L. A., Gitin, Y., Parkinson, J. S. (2000). Domain organization and flavin adenine dinucleotide-binding determinants in the aerotaxis signal transducer Aer of Escherichia coli. Proc. Natl. Acad. Sci. USA 97: 5830-5835 [Abstract] [Full Text]