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J Bacteriol. 1976 November; 128(2): 683-688

Inorganic nitrogen assimilation by the photosynthetic bacterium Rhodopseudomonas capsulata.

B C Johansson and H Gest

ABSTRACT

The photosynthetic bacterium Rhodopseudomonas capsulata lacks glutamate dehydrogenase and normally uses the glutamine synthetase/glutamate synthase sequence of reactions for assimilation of N2 and ammonia. The glutamine synthetase in cell-free extracts of the organism is completely sedimented by centrifugation at 140,000 X g for 2 h, is inhibited by L-alanine but not by adenosine 5'-monophosphate, and exhibits two apparent Km values for ammonia (ca. 13 muM and 1 mM).

J Bacteriol. 1976 November; 128(2): 683-688






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