This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chandler, M Articles by Caro, L Search for Related Content PubMed PubMed Citation Articles by Chandler, M Articles by Caro, L

 Previous Article  |  Next Article 

J Bacteriol. 1977 August; 131(2): 421-430

Suppression of an Escherichia coli dnaA mutation by the integrated R factor R100.1: origin of chromosome replication during exponential growth.

M Chandler, L Silver and L Caro

ABSTRACT

We have investigated the behavior, during exponential growth, of strains of Escherichia coli carrying a dnaA(Ts) mutation that has been suppressed by the integration of the F-like R plasmid R100.1. We present evidence showing that replication in these strains proceeds largely from the normal chromosome origin at 30 degrees C, a permissive temperature for the dnaA(Ts) gene product, whereas, at 42 degrees C, replication proceeds largely from the integrated plasmid. These conclusions are based on measurements made by deoxyribonucleic acid:deoxyribonucleic acid hybridization of the relative frequencies of the prophages Mu-1 and lambdaind- and R100.1 integrated at known locations on the E. coli chromosome in these Hfr strains.

---

J Bacteriol. 1977 August; 131(2): 421-430

This article has been cited by other articles:

• Chandler, M., Clerget, M., Caro, L. (1981). IS1-promoted Events Associated with Drug-resistance Plasmids. Cold Spring Harb Symp Quant Biol 45: 157-165 [Abstract]  
• Chandler, M., Silver, L., Lane, D., Caro, L. (1979). Properties of an Autonomous r-Determinant from R100.1. Cold Spring Harb Symp Quant Biol 43: 1223-1231 [Abstract]