This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Gerdes, R G Articles by Rosenberg, H Search for Related Content PubMed PubMed Citation Articles by Gerdes, R G Articles by Rosenberg, H

 Previous Article  |  Next Article 

J Bacteriol. 1977 August; 131(2): 512-518

Restoration of phosphate transport by the phosphate-binding protein in spheroplasts of Escherichia coli.

ABSTRACT

Reconstitution of phosphate transport in Escherichia coli was demonstrated. Conversion of E. coli K10 cells to spheroplasts decreased phosphate transport to about 2%. Addition of purified phosphate-binding protein at physiological levels to these spheroplasts caused a mean 14-fold increase in phosphate transport rate. Crude shock fluid fractions were also stimulatory but not if the shock fluid was obtained from mutants lacking phosphate-binding protein. The effect of the binding protein was abolished by its specific antibody. The phosphate was shown to have entered the cell, where it became esterified. Reconstitution was not possible with cold-shocked or osmotically shocked cells.