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J Bacteriol. 1977 September; 131(3): 1026-1028

Kinase replacement by a dehydrogenase for Escherichia coli glycerol utilization.

E J St Martin, W B Freedberg and E C Lin

ABSTRACT

A mutant of Escherichia coli that employs a glycerol:nicotinamide adenine dinucleotide 2-oxidoreductase (EC 1.1.1.6), instead of adenosine 5'-triphosphate:glycerol 3-phosphotransferase (EC 2.7.1.30), as the first enzyme for the dissimilation of glycerol was constructed. This mutant, like the wild-type strain, still cannot grow anaerobically on glycerol without an exogenous hydrogen acceptor.


J Bacteriol. 1977 September; 131(3): 1026-1028




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