This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sumrada, R Articles by Cooper, T G Search for Related Content PubMed PubMed Citation Articles by Sumrada, R Articles by Cooper, T G

 Previous Article  |  Next Article 

J Bacteriol. 1977 September; 131(3): 839-847

Allantoin transport in Saccharomyces cerevisiae.

ABSTRACT

Allantoin uptake in both growing and resting cultures of Saccharomyces cerevisiae occurs by a low-Km (ca. 15 micrometer) transport system that uses energy that is likely generated in the cytoplasm. This conclusion was based on the observation that transport did not occur in the absence of glucose or the presence of dinitrophenol, carbonyl cyanide-m-chloro-phenyl hydrazine, fluoride, or arsenate ions. Normal uptake was observed, however, in the presence of cyanide. The rate of accumulation was maximal at pH 5.2. In contrast to the urea transport system, allantoin uptake appeared to be unidirectional. Preloaded, radioactive allantoin was not lost from cells suspended in allantoin-free buffer and did not exchange with exogenously added, nonradioactive allantoin. Treatment of preloaded cells with nystatin, however, released the accumulated radioactivity. Allantoin accumulated within cells was isolated and shown to be chemically unaltered.

This article has been cited by other articles:

• Suzuki, S., Henderson, P. J. F. (2006). The Hydantoin Transport Protein from Microbacterium liquefaciens. J. Bacteriol. 188: 3329-3336 [Abstract] [Full Text]