This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murooka, Y
Right arrow Articles by Harada, T
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murooka, Y
Right arrow Articles by Harada, T

 Previous Article  |  Next Article 

J Bacteriol. 1977 October; 132(1): 247-253

Immunological study of the regulation of cellular arylsulfatase synthesis in Klebsiella aerogenes.

Y Murooka, T Yamada, S Tanabe and T Harada

ABSTRACT

Regulation of cellular arylsulfatase synthesis in Klebsiella aerogenes was analyzed by immunological techniques. Antibody directed against the purified arylsulfatase from K. aerogenes W70 was obtained from rabbits and characterized by immunoelectrophoresis, double-diffusion, quantitative precipitation, and enzyme neutralization tests. Arylsulfatase was located in the periplasmic space when the wild-type strain was cultured with methionine or with inorganic sulfate plus tyramine, but not with inorganic sulfate without tyramine, as the sole sulfur source. Tyramine oxidase was retained in the membrane fraction prepared from cells grown in the presence of tyramine. Arylsulfatase protein was not synthesized in the presence of tyramine and inorganic sulfate by mutant K611, which is deficient in tyramine oxidase (tynA). We conclude that the expression of the arylsulfatase gene (atsA) is regulated by the expression of tynA and that inorganic sulfate serves as a corepressor. In addition, strains mutated in the atsA gene were analyzed by using antibody.

J Bacteriol. 1977 October; 132(1): 247-253




This article has been cited by other articles:

  • Hoffman, J. A., Badger, J. L., Zhang, Y., Huang, S.-H., Kim, K. S. (2000). Escherichia coli K1 aslA Contributes to Invasion of Brain Microvascular Endothelial Cells In Vitro and In Vivo. Infect. Immun. 68: 5062-5067 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»