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J Bacteriol. 1977 October; 132(1): 349-351

Transformation in Escherichia coli: cryogenic preservation of competent cells.

D A Morrison

ABSTRACT

Escherichia coli cells prepared for transformation by treatment with cold 0.1 M CaCl2 remained viable and competent after storage at -82 degrees C in 15% glycerol; thawed-cell samples yielded up to 10(6) transformants per microgram of plasmid deoxyribonucleic acid.

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J Bacteriol. 1977 October; 132(1): 349-351

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