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J Bacteriol. 1977 October; 132(1): 359-361

Localized mutagenesis with bacteriophage Mu: method for increasing the frequency of specific bacterial mutants.

H Tabor, E W Hafner and C W Tabor

ABSTRACT

A method is described for markedly enriching a bacterial population for cells containing any given Mu insertion mutation. The method involves the transfer of a small piece of deoxyribonucleic acid from a Mu-infected Hfr donor donor strain to a suitable F- strain and a subsequent selection of those recombinant organisms that have received a Mu prophage from the donor. The method is particularly usefule for isolating mutants whose selection requires "brute-force" assay, since only a few hundred colonies have to be screened.

J Bacteriol. 1977 October; 132(1): 359-361






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