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J Bacteriol. 1977 December; 132(3): 1036-1037

Selection of yeast auxotrophs by thymidylate starvation.

B J Barclay and J G Little

ABSTRACT

A rapid procedure for the recovery of Saccharomyces cerevisiae auxotrophs was developed by exploiting the protection of these mutants from thymineless death when a required metabolite was withheld. The method can be used for thymidine 5'-monophosphate-requiring auxotrophs or wild-type strains blocked in de novo synthesis of thymidylate by folate antagonists.

J Bacteriol. 1977 December; 132(3): 1036-1037






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