This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gryczan, T J Articles by Dubnau, D Search for Related Content PubMed PubMed Citation Articles by Gryczan, T J Articles by Dubnau, D

 Previous Article  |  Next Article 

J Bacteriol. 1978 April; 134(1): 318-329

Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.

ABSTRACT

Covalently closed circular DNA from five Staphylococcus aureus plasmids has been introduced into Bacillus subtilis. Four of these plasmids (pUB110, pCM194, pSA2100, and pSA0501) have been selected for further study. These plasmids replicate as multicopy autonomous replicons in both Rec+ and Rec- B. subtilis strains. They may be transduced between B. subtilis strains or transformed at a frequency of 10(4) to 10(5) transformants per microgram of DNA. The molecular weights of these plasmids were estimated, and restriction endonuclease cleavage site maps are presented. Evidence is given that pSA2100, an in vivo recombinant of pSA0501 and pCM194 (S. Iordnescu, J. Bacteriol. 124:597-601, 1975), arose by a fusion of the latter plasmids, possibly by insertion of one element into another as a translocatable element. Genetic information from three other S. aureus plasmids (pK545, pSH2, and pUB101) has also been introduced into B. subtilis, although no covalently closed circular plasmid DNA was recovered.

This article has been cited by other articles:

• Sorvig, E., Skaugen, M., Naterstad, K., Eijsink, V. G. H., Axelsson, L. (2005). Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin-antitoxin-like plasmid maintenance system. Microbiology 151: 421-431 [Abstract] [Full Text]  
• Arnaud, M., Chastanet, A., Debarbouille, M. (2004). New Vector for Efficient Allelic Replacement in Naturally Nontransformable, Low-GC-Content, Gram-Positive Bacteria. Appl. Environ. Microbiol. 70: 6887-6891 [Abstract] [Full Text]  
• Airaksinen, U., Penttila, T., Wahlstrom, E., Vuola, J. M., Puolakkainen, M., Sarvas, M. (2003). Production of Chlamydia pneumoniae Proteins in Bacillus subtilis and Their Use in Characterizing Immune Responses in the Experimental Infection Model. CVI 10: 367-375 [Abstract] [Full Text]  
• Darmon, E., Noone, D., Masson, A., Bron, S., Kuipers, O. P., Devine, K. M., Dijl, J. M. v. (2002). A Novel Class of Heat and Secretion Stress-Responsive Genes Is Controlled by the Autoregulated CssRS Two-Component System of Bacillus subtilis. J. Bacteriol. 184: 5661-5671 [Abstract] [Full Text]  
• Stein, T., Borchert, S., Conrad, B., Feesche, J., Hofemeister, B., Hofemeister, J., Entian, K.-D. (2002). Two Different Lantibiotic-Like Peptides Originate from the Ericin Gene Cluster of Bacillus subtilis A1/3. J. Bacteriol. 184: 1703-1711 [Abstract] [Full Text]