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J Bacteriol. 1978 July; 135(1): 133-137

Study of calcium dipicolinate release during bacterial spore germination by using a new, sensitive assay for dipicolinate.

I R Scott and D J Ellar

ABSTRACT

The release of calcium and dipicolinic acid from spores of Bacillus megaterium KM during L-alanine-induced triggering of germination has been studied using a new, simple, and rapid assay for dipicolinic acid capable of detecting a concentration of 0.5 micron. The release of both calcium and dipicolinate started within seconds of exposure of the spores to L-alanine, thus preceding other measurable changes associated with germination. From the earliest times, the two substances were released in equimolar quantities, although later in germination calcium predominated.

J Bacteriol. 1978 July; 135(1): 133-137




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