This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zitomer, R S Articles by Nichols, D L Search for Related Content PubMed PubMed Citation Articles by Zitomer, R S Articles by Nichols, D L

Kinetics of glucose repression of yeast cytochrome c.

ABSTRACT

The kinetics of glucose repression of cytochrome c synthesis was measured by a radioimmune assay. When 5 or 10% glucose was added to a derepressed culture, the rate of cytochrome c synthesis was reduced to the repressed level with a half-life of 2 min. The addition of 1 or 0.5% glucose repressed the rate of cytochrome c synthesis to the same level as high glucose concentrations but with a longer half-life of 3 min. Glucose repression had no effect on the stability or function of the cytochrome c protein. Cellular levels of active cytochrome c mRNA during glucose repression were measured by translation of total cellular polyadenylic acid-containing RNA and immunoprecipitation cytochrome c from the translation products. The results of these measurements indicate that glucose represses the rate of cytochrome c synthesis through a reduction in the level of translatable cytochrome c mRNA.

This article has been cited by other articles:

• Gancedo, J. M. (1998). Yeast Carbon Catabolite Repression. Microbiol. Mol. Biol. Rev. 62: 334-361 [Abstract] [Full Text]  
• Vincent, O., Gancedo, J. M. (1995). Analysis of Positive Elements Sensitive to Glucose in the Promoter of the FBP1 Gene from Yeast. J. Biol. Chem. 270: 12832-12838 [Abstract] [Full Text]