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Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis.

ABSTRACT

Motile, but generally nonchemotactic (che) mutants of Escherichia coli were examined for complementation and recombination with specialized lambdafla transducing phages. The complex complementation behavior of these mutants found previously in F-prime tests could largely be accounted for by intragenic complementation and by polarity effects. Mutants of the "cheA" class defined two genes, cheA and cheW, which appeared to be contranscribed. Mutants of the "cheB" class defined four genes, cheX, cheB, cheY, and cheZ, which also constituted a transcriptional unit. Mutants defective in cheA, cheW, cheX, or cheY function swam smoothly, with little or no tumbling, whereas cheB or cheZ mutants exhibited very high tumbling rates. These functions are probably involved in initiating of controlling changes in flagellar rotation in response to chemotactic stimuli.

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• Alexandre, G., Zhulin, I. B. (2003). Different Evolutionary Constraints on Chemotaxis Proteins CheW and CheY Revealed by Heterologous Expression Studies and Protein Sequence Analysis. J. Bacteriol. 185: 544-552 [Abstract] [Full Text]  
• Boukhvalova, M., VanBruggen, R., Stewart, R. C. (2002). CheA Kinase and Chemoreceptor Interaction Surfaces on CheW. J. Biol. Chem. 277: 23596-23603 [Abstract] [Full Text]  
• Boukhvalova, M. S., Dahlquist, F. W., Stewart, R. C. (2002). CheW Binding Interactions with CheA and Tar. IMPORTANCE FOR CHEMOTAXIS SIGNALING IN ESCHERICHIA COLI. J. Biol. Chem. 277: 22251-22259 [Abstract] [Full Text]  
• Poggio, S., Osorio, A., Corkidi, G., Dreyfus, G., Camarena, L. (2001). The N Terminus of FliM Is Essential To Promote Flagellar Rotation in Rhodobacter sphaeroides. J. Bacteriol. 183: 3142-3148 [Abstract] [Full Text]  
• Maki, N., Gestwicki, J. E., Lake, E. M., Kiessling, L. L., Adler, J. (2000). Motility and Chemotaxis of Filamentous Cells of Escherichia coli. J. Bacteriol. 182: 4337-4342 [Abstract] [Full Text]  
• Samuel, A. D. T., Pitta, T. P., Ryu, W. S., Danese, P. N., Leung, E. C. W., Berg, H. C. (1999). Flagellar determinants of bacterial sensitivity to chi -phage. Proc. Natl. Acad. Sci. USA 96: 9863-9866 [Abstract] [Full Text]  
• Mathews, M. A. A., Tang, H. L., Blair, D. F. (1998). Domain Analysis of the FliM Protein of Escherichia coli. J. Bacteriol. 180: 5580-5590 [Abstract] [Full Text]  
• Scharf, B. E., Fahrner, K. A., Berg, H. C. (1998). CheZ Has No Effect on Flagellar Motors Activated by CheY13DK106YW. J. Bacteriol. 180: 5123-5128 [Abstract] [Full Text]  
• Shukla, D., Zhu, X. Y., Matsumura, P. (1998). Flagellar Motor-switch Binding Face of CheY and the Biochemical Basis of Suppression by CheY Mutants That Compensate for Motor-switch Defects in Escherichia coli. J. Biol. Chem. 273: 23993-23999 [Abstract] [Full Text]  
• Donato, G. M., Kawula, T. H. (1998). Enhanced Binding of Altered H-NS Protein to Flagellar Rotor Protein FliG Causes Increased Flagellar Rotational Speed and Hypermotility in Escherichia coli. J. Biol. Chem. 273: 24030-24036 [Abstract] [Full Text]  
• Abouhamad, W. N., Bray, D., Schuster, M., Boesch, K. C., Silversmith, R. E., Bourret, R. B. (1998). Computer-Aided Resolution of an Experimental Paradox in Bacterial Chemotaxis. J. Bacteriol. 180: 3757-3764 [Abstract] [Full Text]  
• Burkart, M., Toguchi, A., Harshey, R. M. (1998). The chemotaxis system, but not chemotaxis, is essential for swarming motility in Escherichia coli. Proc. Natl. Acad. Sci. USA 95: 2568-2573 [Abstract] [Full Text]  
• Weerasuriya, S., Schneider, B. M., Manson, M. D. (1998). Chimeric Chemoreceptors in Escherichia coli: Signaling Properties of Tar-Tap and Tap-Tar Hybrids. J. Bacteriol. 180: 914-920 [Abstract] [Full Text]  
• Scharf, B. E., Fahrner, K. A., Turner, L., Berg, H. C. (1998). Control of direction of flagellar rotation in bacterial chemotaxis. Proc. Natl. Acad. Sci. USA 95: 201-206 [Abstract] [Full Text]  
• Ottemann, K. M., Koshland, D. E. Jr. (1997). Converting a transmembrane receptor to a soluble receptor: Recognition domain to effector domain signaling after excision of the transmembrane domain. Proc. Natl. Acad. Sci. USA 94: 11201-11204 [Abstract] [Full Text]  
• Zhu, X., Volz, K., Matsumura, P. (1997). The CheZ-binding Surface of CheY Overlaps the CheA- and FliM-binding Surfaces. J. Biol. Chem. 272: 23758-23764 [Abstract] [Full Text]  
• Surette, M. G., Stock, J. B. (1996). Role of alpha -Helical Coiled-coil Interactions in Receptor Dimerization, Signaling, and Adaptation during Bacterial Chemotaxis. J. Biol. Chem. 271: 17966-17973 [Abstract] [Full Text]  
• Blat, Y., Eisenbach, M. (1996). Oligomerization of the Phosphatase CheZ Upon Interaction with the Phosphorylated Form of CheY. J. Biol. Chem. 271: 1226-1231 [Abstract] [Full Text]  
• Russo, A., Koshland, D. Jr (1983). Separation of signal transduction and adaptation functions of the aspartate receptor in bacterial sensing. Science 220: 1016-1020 [Abstract]  
• Bjorkman, A. M., Dunten, P., Sandgren, M. O. J., Dwarakanath, V. N., Mowbray, S. L. (2001). Mutations That Affect Ligand Binding to the Escherichia coli Aspartate Receptor. IMPLICATIONS FOR TRANSMEMBRANE SIGNALING. J. Biol. Chem. 276: 2808-2815 [Abstract] [Full Text]