This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cantwell, S G Articles by Fall, R R Search for Related Content PubMed PubMed Citation Articles by Cantwell, S G Articles by Fall, R R

Biodegradation of acyclic isoprenoids by Pseudomonas species.

ABSTRACT

The ability of various pseudomonads to utilize acyclic isoprenoids as a sole carbon source was investigated. Tests for utilization of acyclic isoprenols such as citronellol and geraniol were complicated by toxic effects of these alcohols, and most species tested were killed by exposure to citronellol or geraniol (0.1%, vol/vol) in liquid culture. In the case of Pseudomonas citronellolis, sensitivity to isoprenols is reduced by prior induction of the isoprenoid degradative pathway via either growth on succinate in the presence of citronellol or growth on citronellic acid. For this species, citronellic acid proved to be the best isoprenoid growth substrate tested. Geraniol utilization as a taxonomic indicator for different subgroups of pseudomonads is discussed. Only a few of the species tested were able to utilize acyclic isoprenoids. Two species which utilize C10 acyclic isoprenoids, P. aeruginosa and P. mendocina, were shown to contain the inducible enzyme geranyl-coenzyme A carboxylase, one of the unique enzymes in the isoprenol degradative pathway known to occur in P. citronellolis. Of the species which utilized geranitol, none showed definite growth on the homologous C15 and C20 isoprenols.

This article has been cited by other articles:

• Forster-Fromme, K., Chattopadhyay, A., Jendrossek, D. (2008). Biochemical characterization of AtuD from Pseudomonas aeruginosa, the first member of a new subgroup of acyl-CoA dehydrogenases with specificity for citronellyl-CoA. Microbiology 154: 789-796 [Abstract] [Full Text]  
• Forster-Fromme, K., Hoschle, B., Mack, C., Bott, M., Armbruster, W., Jendrossek, D. (2006). Identification of Genes and Proteins Necessary for Catabolism of Acyclic Terpenes and Leucine/Isovalerate in Pseudomonas aeruginosa.. Appl. Environ. Microbiol. 72: 4819-4828 [Abstract] [Full Text]  
• Hoschle, B., Gnau, V., Jendrossek, D. (2005). Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved in leucine/isovalerate utilization (Liu) and acyclic terpene utilization (Atu), and are encoded by liuB/liuD and atuC/atuF, in Pseudomonas aeruginosa. Microbiology 151: 3649-3656 [Abstract] [Full Text]  
• Hoschle, B., Jendrossek, D. (2005). Utilization of geraniol is dependent on molybdenum in Pseudomonas aeruginosa: evidence for different metabolic routes for oxidation of geraniol and citronellol. Microbiology 151: 2277-2283 [Abstract] [Full Text]  
• Berekaa, M. M., Steinbüchel, A. (2000). Microbial Degradation of the Multiply Branched Alkane 2,6,10,15,19,23-Hexamethyltetracosane (Squalane) by Mycobacterium fortuitum and Mycobacterium ratisbonense. Appl. Environ. Microbiol. 66: 4462-4467 [Abstract] [Full Text]  
• McKean, A. L., Ke, J., Song, J., Che, P., Achenbach, S., Nikolau, B. J., Wurtele, E. S. (2000). Molecular Characterization of the Non-biotin-containing Subunit of 3-Methylcrotonyl-CoA Carboxylase. J. Biol. Chem. 275: 5582-5590 [Abstract] [Full Text]  
• Rontani, J.-F., Bonin, P. C., Volkman, J. K. (1999). Biodegradation of Free Phytol by Bacterial Communities Isolated from Marine Sediments under Aerobic and Denitrifying Conditions. Appl. Environ. Microbiol. 65: 5484-5492 [Abstract] [Full Text]  
• Rontani, J.-F., Bonin, P. C., Volkman, J. K. (1999). Production of Wax Esters during Aerobic Growth of Marine Bacteria on Isoprenoid Compounds. Appl. Environ. Microbiol. 65: 221-230 [Abstract] [Full Text]