This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Johnson, R C Search for Related Content PubMed PubMed Citation Articles by Johnson, R C

Reduction of postreplication DNA repair in two Escherichia coli mutants with temperature-sensitive polymerase III activity: implications for the postreplication repair pathway.

ABSTRACT

Daughter strand gaps are secondary lesions caused by interrupted DNA synthesis in the proximity of UV-induced pyrimidine dimers. The relative roles of DNA recombination and de novo DNA synthesis in filling such gaps have not been clarified, although both are required for complete closure. In this study, the Escherichia coli E486 and E511 dnaE(Ts) mutants, in which DNA polymerase I but not DNA polymerase III is active at 43 degrees C, were examined. Both mutants demonstrated reduced gap closure in comparison with the progenitor strain at the nonpermissive temperature. These results and those of previous studies support the hypothesis that both DNA polymerase I and DNA polymerase III contribute to gap closure, suggesting a cooperative effort in the repair of each gap. Benzoylated, naphthoylated diethylaminoethyl-cellulose chromatography analysis for persistence of single-strand DNA in the absence of DNA polymerase III activity suggested that de novo DNA synthesis initiates the filling of daughter strand gaps.

This article has been cited by other articles:

• Maul, R. W., Sanders, L. H., Lim, J. B., Benitez, R., Sutton, M. D. (2007). Role of Escherichia coli DNA Polymerase I in Conferring Viability upon the dnaN159 Mutant Strain. J. Bacteriol. 189: 4688-4695 [Abstract] [Full Text]  
• Kuzminov, A. (1999). Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage lambda. Microbiol. Mol. Biol. Rev. 63: 751-813 [Abstract] [Full Text]