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J Bacteriol. 1978 October; 136(1): 441-443

Lack of lysogenic induction in "diaminopimelic acid spheroplasts".

A Bailone and A Levine

ABSTRACT

As part of an attempt to develop a semi-in vitro system of lysogenic induction, using spheroplasts of Escherichia coli K-12 lysogenic for prophage lambda, we prepared spheroplasts by depriving E. coli dap of diaminopimelic acid (DAP-spheroplasts). DAP-spheroplasts made from E. coli (lambda cI857) were thermally inducible. However, DAP-spheroplasts of E. coli (lambda) were not inducible by UV light. Thus, it appears that a functional cell wall is required for UV induction of prophage lambda.

J Bacteriol. 1978 October; 136(1): 441-443






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