This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pramanik, A Articles by Schulz, H Search for Related Content PubMed PubMed Citation Articles by Pramanik, A Articles by Schulz, H

 Previous Article  |  Next Article 

J Bacteriol. 1979 January; 137(1): 469-473

Five different enzymatic activities are associated with the multienzyme complex of fatty acid oxidation from Escherichia coli.

ABSTRACT

The purified multienzyme complex of fatty acid oxidation from Escherichia coli was found to possess 3-hydroxyacyl-coenzyme A (CoA) epimerase and cis-delta3-trans-delta2-enoyl-CoA isomerase activities in addition to the previously identified enoyl-CoA hydratase, L-3-hydroxyacyl-CoA dehydrogenase, and 3-ketoactyl-CoA thiolase activities. Evidence is presented in support of the proposed association of all five enzyme activities with one protein which apparently is composed of two types of subunits and which can exist in several aggregated forms. The five component enzymes of the complex were rapidly inactivated by tris(hydroxymethyl)aminomethane, whereas they remained active in the presence of potassium phosphate.

This article has been cited by other articles:

• Son, M. S., Matthews, W. J. Jr., Kang, Y., Nguyen, D. T., Hoang, T. T. (2007). In Vivo Evidence of Pseudomonas aeruginosa Nutrient Acquisition and Pathogenesis in the Lungs of Cystic Fibrosis Patients. Infect. Immun. 75: 5313-5324 [Abstract] [Full Text]  
• Cho, B.-K., Knight, E. M., Palsson, B. O. (2006). Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA.. Microbiology 152: 2207-2219 [Abstract] [Full Text]  
• Snell, K. D., Feng, F., Zhong, L., Martin, D., Madison, L. L. (2002). YfcX Enables Medium-Chain-Length Poly(3-Hydroxyalkanoate) Formation from Fatty Acids in Recombinant Escherichia coli fadB Strains. J. Bacteriol. 184: 5696-5705 [Abstract] [Full Text]