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J Bacteriol. 1979 April; 138(1): 257-260

Bidirectional replication of the mini-ColE1 plasmid pVH51.

R Eichenlaub, R B Sparks and D R Helinski

ABSTRACT

Replicating molecules of the min-ColE1 plasmid pVH51 have been examined by electron microscopy after cleavage with the restriction endonuclease EcoRI. Replication apparently starts at a unique site indistinguishable from the origin of replication used by the parental plasmid ColE1. In contrast to ColE1, the structure of the majority of the replication intermediates was consistent with a bidirectional mode of replication. A minor portion of the molecules appeared to replicate unidirectionally in either direction from the same origin.

J Bacteriol. 1979 April; 138(1): 257-260






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