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J Bacteriol. 1979 June; 138(3): 779-782

Penicillin selection of Escherichia coli deoxyribonucleic acid repair mutants.

A Sancar and C S Rupert

ABSTRACT

A rapid method has been developed for isolation of ultraviolet-sensitive mutants of Escherichia coli, by inducing delay in the growth and/or division of repair-deficienct cells with low fluences of far-ultraviolet radiation, and killing with penicillin the repair-proficient cells, which continue to grow and divide. With this technique, we have achieved about a 3,000-fold enrichment for photoreactivation less (phr) cells and have isolated and characterized three phr mutants.

J Bacteriol. 1979 June; 138(3): 779-782




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