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J Bacteriol. 1979 July; 139(1): 316-317

General transduction in Rhizobium meliloti by a thermosensitive mutant of bacteriophage DF2.

J Casadesús and J Olivares

ABSTRACT

A simiplified version of bacteriophage DF2 transduction, using the thermosensitive mutant DF2tsC, is described. The transduction frequencies of Rhizobium meliloti markers by DF1tsC are of about 10(-6) or higher. The development of transductants at 34 degrees C does not require the addition of antiserum for phage neutralization.

J Bacteriol. 1979 July; 139(1): 316-317






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Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
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