This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Winkler, M E Search for Related Content PubMed PubMed Citation Articles by Winkler, M E

Ribosomal ribonucleic acid isolated from Salmonella typhimurium: absence of the intact 23S species.

ABSTRACT

Ribonucleic acid (RNA) isolated by four distinct methods and from a variety of Salmonella typhimurium strains lacked intact 23S ribosomal RNA (rRNA). On sucrose gradients which minimize aggregation, the vast majority of S. typhimurium rRNA sedimented as a 16S peak with a 14S shoulder. RNA from this region of the gradient was resolved into three discrete bands by electrophoresis in formamide. Two very minor S. typhimurium RNA peaks were resolved at 21S and 10S on sucrose gradients, and each peak formed discrete bands in electrophoresis. It is concluded that if S. typhimurium does possess an intact 23S rRNA species, this species is extremely "labile." The absence of isolatable S. typhimurium 23S rRNA possibly reflected in vivo processing of the rRNA before isolation. Under certain conditions, S. typhimurium rRNA formed discrete aggregates which sedimented similarly to intact Escherichia coli 23S rRNA.

This article has been cited by other articles:

• Bouchet, V., Huot, H., Goldstein, R. (2008). Molecular Genetic Basis of Ribotyping. Clin. Microbiol. Rev. 21: 262-273 [Abstract] [Full Text]  
• van Berkum, P., Terefework, Z., Paulin, L., Suomalainen, S., Lindstrom, K., Eardly, B. D. (2003). Discordant Phylogenies within the rrn Loci of Rhizobia. J. Bacteriol. 185: 2988-2998 [Abstract] [Full Text]  
• van Berkum, P., Eardly, B. D. (2002). The Aquatic Budding Bacterium Blastobacter denitrificans Is a Nitrogen-Fixing Symbiont of Aeschynomene indica. Appl. Environ. Microbiol. 68: 1132-1136 [Abstract] [Full Text]  
• Pronk, L. M., Sanderson, K. E. (2001). Intervening Sequences in rrl Genes and Fragmentation of 23S rRNA in Genera of the Family Enterobacteriaceae. J. Bacteriol. 183: 5782-5787 [Abstract] [Full Text]  
• Miller, W. L., Pabbaraju, K., Sanderson, K. E. (2000). Fragmentation of 23S rRNA in Strains of Proteus and Providencia Results from Intervening Sequences in the rrn (rRNA) Genes. J. Bacteriol. 182: 1109-1117 [Abstract] [Full Text]