This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shapiro, S Articles by Wolfe, R S Search for Related Content PubMed PubMed Citation Articles by Shapiro, S Articles by Wolfe, R S

 Previous Article  |  Next Article 

J Bacteriol. 1980 February; 141(2): 728-734

Methyl-coenzyme M, an intermediate in methanogenic dissimilation of C1 compounds by Methanosarcina barkeri.

ABSTRACT

Extracts of Methanosarcina barkeri possess a specific methyltransferase that catalyzes the transfer of the methyl group of methanol to 2-mercaptoethanesulfonic acid. Over a fourfold range in added 2-mercaptoethanesulfonic acid, the formation of 2-(methylthio)ethanesulfonic acid exhibited a 1:1 ratio to 2-mercaptoethanesulfonic acid added. This reaction required adenosine 5'-triphosphate; a maximal ratio (mole/mole) of 85 methyl groups was transferred per adenosine 5'-triphosphate added. The methyltransferase was found in extracts of methanol-grown cells as well as in extracts of hydrogen-grown cells. In extracts of cells grown on either substrate, 2-(methylthio)ethanesulfonic acid was formed from added methanol or methylamine but not from acetate.

This article has been cited by other articles:

• Zeikus, J., Kerby, R, Krzycki, J. (1985). Single-carbon chemistry of acetogenic and methanogenic bacteria. Science 227: 1167-1173 [Abstract]  
• Wood, J., Moura, I, Moura, J., Santos, M., Xavier, A., LeGall, J, Scandellari, M (1982). Role of vitamin B12 in methyl transfer for methane biosynthesis by Methanosarcina barkeri. Science 216: 303-305 [Abstract]