This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Goldie, A H Articles by Sanwal, B D Search for Related Content PubMed PubMed Citation Articles by Goldie, A H Articles by Sanwal, B D

 Previous Article  |  Next Article 

J Bacteriol. 1980 March; 141(3): 1115-1121

Genetic and physiological characterization of Escherichia coli mutants deficient in phosphoenolpyruvate carboxykinase activity.

ABSTRACT

Mutants doubly deficient in phosphoenolpyruvate carboxykinase (pck) and phosphoenolpyruvate synthetase (pps) were unable to grow with succinate as the sole carbon source. A number of pck mutations isolated from pps strains by penicillin selection mapped at 74 min on the Escherichia coli chromosome, between glpD and aroB. Several of the strains containing these mutations had a protein antigenically related to phosphoenolpyruvate carboxykinase, and therefore, the mutations probably represented mutations in the structural gene for this enzyme. Phosphoenolpyruvate carboxykinase was regulated at the level of transcription by catabolite repression. Enzyme levels also increased in stationary-phase cultures by a mechanism independent of cyclic adenosine monophosphate or the product of the relA gene.

This article has been cited by other articles:

• Miranda, R. L., Conway, T., Leatham, M. P., Chang, D. E., Norris, W. E., Allen, J. H., Stevenson, S. J., Laux, D. C., Cohen, P. S. (2004). Glycolytic and Gluconeogenic Growth of Escherichia coli O157:H7 (EDL933) and E. coli K-12 (MG1655) in the Mouse Intestine. Infect. Immun. 72: 1666-1676 [Abstract] [Full Text]  
• Velayudhan, J., Kelly, D. J. (2002). Analysis of gluconeogenic and anaplerotic enzymes in Campylobacter jejuni: an essential role for phosphoenolpyruvate carboxykinase. Microbiology 148: 685-694 [Abstract] [Full Text]  
• Hausler, R. E., Rademacher, T., Li, J., Lipka, V., Fischer, K. L., Schubert, S., Kreuzaler, F., Hirsch, H.-J. (2001). Single and double overexpression of C4-cycle genes had differential effects on the pattern of endogenous enzymes, attenuation of photorespiration and on contents of UV protectants in transgenic potato and tobacco plants. J Exp Bot 52: 1785-1803 [Abstract] [Full Text]  
• Inui, M., Nakata, K., Roh, J. H., Zahn, K., Yukawa, H. (1999). Molecular and Functional Characterization of the Rhodopseudomonas palustris No. 7 Phosphoenolpyruvate Carboxykinase Gene. J. Bacteriol. 181: 2689-2696 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
• Matte, A., Tari, L. W., Goldie, H., Delbaere, L. T.J. (1997). Structure and Mechanism of Phosphoenolpyruvate Carboxykinase. J. Biol. Chem. 272: 8105-8108 [Full Text]