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J Bacteriol. 1980 May; 142(2): 508-512

Plasmid-mediated transformation in Bacillus megaterium.

B J Brown and B C Carlton

ABSTRACT

A transformation system was developed for Bacillus megaterium by using antibiotic resistance plasmid deoxyribonucleic acid molecules derived from Staphylococcus aureus and Bacillus cereus. Lysozyme-generated protoplasts of B. megaterium allowed uptake of plasmid deoxyribonucleic acid in the presence of polyethylene glycol. Transformants expressed the antibiotic resistance determinants present on the plasmid deoxyribonucleic acid, and reisolated plasmid deoxyribonucleic acid yielded restriction endonuclease digestion patterns identical to those of the donor deoxyribonucleic acid.

J Bacteriol. 1980 May; 142(2): 508-512






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