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J Bacteriol. 1980 December; 144(3): 877-883

Formation of a lambda (Tn10) tyrR+ specialized transducing bacteriophage from Escherichia coli K-12.

C S Cobbett and J Pittard

ABSTRACT

The transposon Tn10, coding for resistance to tetracycline, was inserted close to the tyrR+ gene at min 28 on the Escherichia coli chromosome. The homology between this transposon and a lambda (Tn10) phage was employed to direct integration of lambda close to tyrR+ with subsequent isolation of a lambda (Tn10) tyrR+ transducing phage. Results of restriction endonuclease analysis of the transducing phage are presented.

J Bacteriol. 1980 December; 144(3): 877-883




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