This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schmidt, L S Articles by Park, J T Search for Related Content PubMed PubMed Citation Articles by Schmidt, L S Articles by Park, J T

 Previous Article  |  Next Article 

J Bacteriol. 1981 January; 145(1): 632-637

Effects of furazlocillin, a beta-lactam antibiotic which binds selectively to penicillin-binding protein 3, on Escherichia coli mutants deficient in other penicillin-binding proteins.

ABSTRACT

Furazlocillin binds selectively to penicillin-binding protein 3 (PBP-3), prevents septation of Escherichia coli, and allows the cells to form long filaments without lysis. The effect of furazlocillin on the morphology, autolysis, and murein synthesis of E. coli mutants deficient in either PBP-1A, PBP-1Bs, or PBP-2 was studied. The results reveal that PBP-1A and PBP-1Bs functions are not equivalent since furazlocillin affects the morphology, autolysis, and murein synthesis of PBP1A- mutants quite differently from that of PBP-1Bs mutants. Different "PBP-2-" mutants were found to respond to furazlocillin in dramatically different ways: strain LS-1 cells formed elongated rods with a central bulge which eventually lysed, whereas SP6 cells formed stable "barbells" in which the two daughter cells were well separated but remained connected by a thick central region.

This article has been cited by other articles:

• Schiffer, G., Holtje, J.-V. (1999). Cloning and Characterization of PBP 1C, a Third Member of the Multimodular Class A Penicillin-binding Proteins of Escherichia coli. J. Biol. Chem. 274: 32031-32039 [Abstract] [Full Text]  
• Denome, S. A., Elf, P. K., Henderson, T. A., Nelson, D. E., Young, K. D. (1999). Escherichia coli Mutants Lacking All Possible Combinations of Eight Penicillin Binding Proteins: Viability, Characteristics, and Implications for Peptidoglycan Synthesis. J. Bacteriol. 181: 3981-3993 [Abstract] [Full Text]  
• Vollmer, W., von Rechenberg, M., Holtje, J.-V. (1999). Demonstration of Molecular Interactions between the Murein Polymerase PBP1B, the Lytic Transglycosylase MltA, and the Scaffolding Protein MipA of Escherichia coli. J. Biol. Chem. 274: 6726-6734 [Abstract] [Full Text]