This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kado, C I Articles by Liu, S T Search for Related Content PubMed PubMed Citation Articles by Kado, C I Articles by Liu, S T

Rapid procedure for detection and isolation of large and small plasmids.

ABSTRACT

Procedures are described for the detection and isolation of plasmids of various sizes (2.6 to 350 megadaltons) that are harbored in species of Agrobacterium, Rhizobium, Escherichia, Salmonella, Erwinia, Pseudomonas, and Xanthomonas. The method utilized the molecular characteristics of covalently closed circular deoxyribonucleic acid (DNA) that is released from cells under conditions that denature chromosomal DNA by using alkaline sodium dodecyl sulfate (pH 12.6) at elevated temperatures. Proteins and cell debris were removed by extraction with phenol-chloroform. Under these conditions chromosomal DNA concentrations were reduced or eliminated. The clarified extract was used directly for electrophoretic analysis. These procedures also permitted the selective isolation of plasmid DNA that can be used directly in nick translation, restriction endonuclease analysis, transformation, and DNA cloning experiments.

This article has been cited by other articles:

• Lin, M.-H., Liu, S.-T. (2008). Stabilization of pSW100 from Pantoea stewartii by the F Conjugation System. J. Bacteriol. 190: 3681-3689 [Abstract] [Full Text]  
• Reynaud, Y., Saulnier, D., Mazel, D., Goarant, C., Le Roux, F. (2008). Correlation between Detection of a Plasmid and High-Level Virulence of Vibrio nigripulchritudo, a Pathogen of the Shrimp Litopenaeus stylirostris. Appl. Environ. Microbiol. 74: 3038-3047 [Abstract] [Full Text]  
• Berk, S. G., Faulkner, G., Garduno, E., Joy, M. C., Ortiz-Jimenez, M. A., Garduno, R. A. (2008). Packaging of Live Legionella pneumophila into Pellets Expelled by Tetrahymena spp. Does Not Require Bacterial Replication and Depends on a Dot/Icm-Mediated Survival Mechanism. Appl. Environ. Microbiol. 74: 2187-2199 [Abstract] [Full Text]  
• Heithoff, D. M., Shimp, W. R., Lau, P. W., Badie, G., Enioutina, E. Y., Daynes, R. A., Byrne, B. A., House, J. K., Mahan, M. J. (2008). Human Salmonella Clinical Isolates Distinct from Those of Animal Origin. Appl. Environ. Microbiol. 74: 1757-1766 [Abstract] [Full Text]  
• Aschbacher, R., Doumith, M., Livermore, D. M., Larcher, C., Woodford, N. (2008). Linkage of acquired quinolone resistance (qnrS1) and metallo-{beta}-lactamase (blaVIM-1) genes in multiple species of Enterobacteriaceae from Bolzano, Italy. J Antimicrob Chemother 61: 515-523 [Abstract] [Full Text]  
• Schenk, A., Weingart, H., Ullrich, M. S. (2008). The alternative sigma factor AlgT, but not alginate synthesis, promotes in planta multiplication of Pseudomonas syringae pv. glycinea. Microbiology 154: 413-421 [Abstract] [Full Text]  
• Wu, G., Carter, B., Mafura, M., Liebana, E., Woodward, M. J., Anjum, M. F. (2008). Genetic Diversity among Escherichia coli O157:H7 Isolates and Identification of Genes Linked to Human Infections. Infect. Immun. 76: 845-856 [Abstract] [Full Text]  
• Carattoli, A., Garcia-Fernandez, A., Varesi, P., Fortini, D., Gerardi, S., Penni, A., Mancini, C., Giordano, A. (2008). Molecular Epidemiology of Escherichia coli Producing Extended-Spectrum -Lactamases Isolated in Rome, Italy. J. Clin. Microbiol. 46: 103-108 [Abstract] [Full Text]  
• Van, T. T. H., Moutafis, G., Tran, L. T., Coloe, P. J. (2007). Antibiotic Resistance in Food-Borne Bacterial Contaminants in Vietnam. Appl. Environ. Microbiol. 73: 7906-7911 [Abstract] [Full Text]  
• Akiba, M., Nakaoka, Y., Kida, M., Ishioka, Y., Sameshima, T., Yoshii, N., Nakazawa, M., Uchida, I., Terakado, N. (2007). Changes in antimicrobial susceptibility in a population of Salmonella enterica serovar Dublin isolated from cattle in Japan from 1976 to 2005. J Antimicrob Chemother 60: 1235-1242 [Abstract] [Full Text]  
• Avsaroglu, M. D., Helmuth, R., Junker, E., Hertwig, S., Schroeter, A., Akcelik, M., Bozoglu, F., Guerra, B. (2007). Plasmid-mediated quinolone resistance conferred by qnrS1 in Salmonella enterica serovar Virchow isolated from Turkish food of avian origin. J Antimicrob Chemother 60: 1146-1150 [Abstract] [Full Text]  
• Chiu, Y.-F., Tung, C.-P., Lee, Y.-H., Wang, W.-H., Li, C., Hung, J.-Y., Wang, C.-Y., Kawaguchi, Y., Liu, S.-T. (2007). A comprehensive library of mutations of Epstein Barr virus. J. Gen. Virol. 88: 2463-2472 [Abstract] [Full Text]  
• Nogrady, N., Toth, A., Kostyak, A., Paszti, J., Nagy, B. (2007). Emergence of multidrug-resistant clones of Salmonella Infantis in broiler chickens and humans in Hungary. J Antimicrob Chemother 60: 645-648 [Abstract] [Full Text]  
• Yamane, K., Wachino, J.-i., Suzuki, S., Kimura, K., Shibata, N., Kato, H., Shibayama, K., Konda, T., Arakawa, Y. (2007). New Plasmid-Mediated Fluoroquinolone Efflux Pump, QepA, Found in an Escherichia coli Clinical Isolate. Antimicrob. Agents Chemother. 51: 3354-3360 [Abstract] [Full Text]  
• Nwaneshiudu, A. I., Mucci, T., Pickard, D. J., Okeke, I. N. (2007). A Second Large Plasmid Encodes Conjugative Transfer and Antimicrobial Resistance in O119:H2 and Some Typical O111 Enteropathogenic Escherichia coli Strains. J. Bacteriol. 189: 6074-6079 [Abstract] [Full Text]  
• Cooke, F. J., Wain, J., Fookes, M., Ivens, A., Thomson, N., Brown, D. J., Threlfall, E. J., Gunn, G., Foster, G., Dougan, G. (2007). Prophage Sequences Defining Hot Spots of Genome Variation in Salmonella enterica Serovar Typhimurium Can Be Used To Discriminate between Field Isolates. J. Clin. Microbiol. 45: 2590-2598 [Abstract] [Full Text]  
• Hopkins, K. L., Wootton, L., Day, M. R., Threlfall, E. J. (2007). Plasmid-mediated quinolone resistance determinant qnrS1 found in Salmonella enterica strains isolated in the UK. J Antimicrob Chemother 59: 1071-1075 [Abstract] [Full Text]  
• Ponciano, J. M., De Gelder, L., Top, E. M., Joyce, P. (2007). The Population Biology of Bacterial Plasmids: A Hidden Markov Model Approach. Genetics 176: 957-968 [Abstract] [Full Text]  
• Talukder, K. A., Mondol, A. S., Islam, M. A., Islam, Z., Dutta, D. K., Khajanchi, B. K., Azmi, I. J., Hossain, M. A., Rahman, M., Cheasty, T., Cravioto, A., Nair, G. B., Sack, D. A. (2007). A novel serovar of Shigella dysenteriae from patients with diarrhoea in Bangladesh. J Med Microbiol 56: 654-658 [Abstract] [Full Text]  
• Vo, A. T. T., van Duijkeren, E., Fluit, A. C., Gaastra, W. (2007). A novel Salmonella genomic island 1 and rare integron types in Salmonella Typhimurium isolates from horses in The Netherlands. J Antimicrob Chemother 59: 594-599 [Abstract] [Full Text]  
• Antunes, P., Machado, J., Peixe, L. (2007). Dissemination of sul3-Containing Elements Linked to Class 1 Integrons with an Unusual 3' Conserved Sequence Region among Salmonella Isolates. Antimicrob. Agents Chemother. 51: 1545-1548 [Abstract] [Full Text]  
• Doi, Y., de Oliveira Garcia, D., Adams, J., Paterson, D. L. (2007). Coproduction of Novel 16S rRNA Methylase RmtD and Metallo-{beta}-Lactamase SPM-1 in a Panresistant Pseudomonas aeruginosa Isolate from Brazil. Antimicrob. Agents Chemother. 51: 852-856 [Abstract] [Full Text]  
• Santander, J., Wanda, S.-Y., Nickerson, C. A., Curtiss, R. III (2007). Role of RpoS in Fine-Tuning the Synthesis of Vi Capsular Polysaccharide in Salmonella enterica Serotype Typhi. Infect. Immun. 75: 1382-1392 [Abstract] [Full Text]  
• Restieri, C., Garriss, G., Locas, M.-C., Dozois, C. M. (2007). Autotransporter-Encoding Sequences Are Phylogenetically Distributed among Escherichia coli Clinical Isolates and Reference Strains. Appl. Environ. Microbiol. 73: 1553-1562 [Abstract] [Full Text]  
• Ma, Z., Smith, J. J., Zhao, Y., Jackson, R. W., Arnold, D. L., Murillo, J., Sundin, G. W. (2007). Phylogenetic Analysis of the pPT23A Plasmid Family of Pseudomonas syringae. Appl. Environ. Microbiol. 73: 1287-1295 [Abstract] [Full Text]  
• De Gelder, L., Ponciano, J. M., Joyce, P., Top, E. M. (2007). Stability of a promiscuous plasmid in different hosts: no guarantee for a long-term relationship. Microbiology 153: 452-463 [Abstract] [Full Text]  
• Valenzuela, J. K., Thomas, L., Partridge, S. R., van der Reijden, T., Dijkshoorn, L., Iredell, J. (2007). Horizontal Gene Transfer in a Polyclonal Outbreak of Carbapenem-Resistant Acinetobacter baumannii. J. Clin. Microbiol. 45: 453-460 [Abstract] [Full Text]  
• Lowell, J. L., Zhansarina, A., Yockey, B., Meka-Mechenko, T., Stybayeva, G., Atshabar, B., Nekrassova, L., Tashmetov, R., Kenghebaeva, K., Chu, M. C., Kosoy, M., Antolin, M. F., Gage, K. L. (2007). Phenotypic and molecular characterizations of Yersinia pestis isolates from Kazakhstan and adjacent regions. Microbiology 153: 169-177 [Abstract] [Full Text]  
• Gaind, R., Paglietti, B., Murgia, M., Dawar, R., Uzzau, S., Cappuccinelli, P., Deb, M., Aggarwal, P., Rubino, S. (2006). Molecular characterization of ciprofloxacin-resistant Salmonella enterica serovar Typhi and Paratyphi A causing enteric fever in India. J Antimicrob Chemother 58: 1139-1144 [Abstract] [Full Text]  
• Schenk, A., Berger, M., Keith, L. M., Bender, C. L., Muskhelishvili, G., Ullrich, M. S. (2006). The algT Gene of Pseudomonas syringae pv. glycinea and New Insights into the Transcriptional Organization of the algT-muc Gene Cluster. J. Bacteriol. 188: 8013-8021 [Abstract] [Full Text]  
• Miyazaki, R., Sato, Y., Ito, M., Ohtsubo, Y., Nagata, Y., Tsuda, M. (2006). Complete Nucleotide Sequence of an Exogenously Isolated Plasmid, pLB1, Involved in {gamma}-Hexachlorocyclohexane Degradation. Appl. Environ. Microbiol. 72: 6923-6933 [Abstract] [Full Text]  
• Kang, M.-S., Besser, T. E., Hancock, D. D., Porwollik, S., McClelland, M., Call, D. R. (2006). Identification of Specific Gene Sequences Conserved in Contemporary Epidemic Strains of Salmonella enterica. Appl. Environ. Microbiol. 72: 6938-6947 [Abstract] [Full Text]  
• Charoenpanich, J., Tani, A., Moriwaki, N., Kimbara, K., Kawai, F. (2006). Dual regulation of a polyethylene glycol degradative operon by AraC-type and GalR-type regulators in Sphingopyxis macrogoltabida strain 103.. Microbiology 152: 3025-3034 [Abstract] [Full Text]  
• Haines, A. S., Akhtar, P., Stephens, E. R., Jones, K., Thomas, C. M., Perkins, C. D., Williams, J. R., Day, M. J., Fry, J. C. (2006). Plasmids from freshwater environments capable of IncQ retrotransfer are diverse and include pQKH54, a new IncP-1 subgroup archetype.. Microbiology 152: 2689-2701 [Abstract] [Full Text]  
• Jin, Y., Ling, J. M. (2006). CTX-M-producing Salmonella spp. in Hong Kong: an emerging problem.. J Med Microbiol 55: 1245-1250 [Abstract] [Full Text]  
• Talukder, K. A., Islam, Z., Dutta, D. K., Islam, M. A., Khajanchi, B. K., Azmi, I. J., Iqbal, M. S., Hossain, M. A., Faruque, A. S. G., Nair, G. B., Sack, D. A. (2006). Antibiotic resistance and genetic diversity of Shigella sonnei isolated from patients with diarrhoea between 1999 and 2003 in Bangladesh.. J Med Microbiol 55: 1257-1263 [Abstract] [Full Text]  
• Sheng, H., Lim, J. Y., Knecht, H. J., Li, J., Hovde, C. J. (2006). Role of Escherichia coli O157:H7 Virulence Factors in Colonization at the Bovine Terminal Rectal Mucosa.. Infect. Immun. 74: 4685-4693 [Abstract] [Full Text]  
• Sidjabat, H. E., Townsend, K. M., Lorentzen, M., Gobius, K. S., Fegan, N., Chin, J. J.-C., Bettelheim, K. A., Hanson, N. D., Bensink, J. C., Trott, D. J. (2006). Emergence and spread of two distinct clonal groups of multidrug-resistant Escherichia coli in a veterinary teaching hospital in Australia.. J Med Microbiol 55: 1125-1134 [Abstract] [Full Text]  
• Struble, J. M., Gill, R. T. (2006). Reverse Engineering Antibiotic Sensitivity in a Multidrug-Resistant Pseudomonas aeruginosa Isolate.. Antimicrob. Agents Chemother. 50: 2506-2515 [Abstract] [Full Text]  
• Kang, M.-S., Besser, T. E., Call, D. R. (2006). Variability in the Region Downstream of the blaCMY-2 {beta}-Lactamase Gene in Escherichia coli and Salmonella enterica Plasmids.. Antimicrob. Agents Chemother. 50: 1590-1593 [Abstract] [Full Text]  
• Sabri, M., Leveille, S., Dozois, C. M. (2006). A SitABCD homologue from an avian pathogenic Escherichia coli strain mediates transport of iron and manganese and resistance to hydrogen peroxide.. Microbiology 152: 745-758 [Abstract] [Full Text]  
• Su, L.-H., Chen, H.-L., Chia, J.-H., Liu, S.-Y., Chu, C., Wu, T.-L., Chiu, C.-H. (2006). Distribution of a transposon-like element carrying blaCMY-2 among Salmonella and other Enterobacteriaceae. J Antimicrob Chemother 57: 424-429 [Abstract] [Full Text]  
• Wachino, J.-i., Kurokawa, H., Suzuki, S., Yamane, K., Shibata, N., Kimura, K., Ike, Y., Arakawa, Y. (2006). Horizontal Transfer of blaCMY-Bearing Plasmids among Clinical Escherichia coli and Klebsiella pneumoniae Isolates and Emergence of Cefepime-Hydrolyzing CMY-19. Antimicrob. Agents Chemother. 50: 534-541 [Abstract] [Full Text]  
• Bartra, S. S., Jackson, M. W., Ross, J. A., Plano, G. V. (2006). Calcium-Regulated Type III Secretion of Yop Proteins by an Escherichia coli hha Mutant Carrying a Yersinia pestis pCD1 Virulence Plasmid. Infect. Immun. 74: 1381-1386 [Abstract] [Full Text]  
• Wachino, J.-i., Yamane, K., Shibayama, K., Kurokawa, H., Shibata, N., Suzuki, S., Doi, Y., Kimura, K., Ike, Y., Arakawa, Y. (2006). Novel Plasmid-Mediated 16S rRNA Methylase, RmtC, Found in a Proteus mirabilis Isolate Demonstrating Extraordinary High-Level Resistance against Various Aminoglycosides. Antimicrob. Agents Chemother. 50: 178-184 [Abstract] [Full Text]  
• Herrero, A., Rodicio, M. R., Gonzalez-Hevia, M. A., Mendoza, M. C. (2006). Molecular epidemiology of emergent multidrug-resistant Salmonella enterica serotype Typhimurium strains carrying the virulence resistance plasmid pUO-StVR2. J Antimicrob Chemother 57: 39-45 [Abstract] [Full Text]  
• Kassis-Chikhani, N., Decre, D., Gautier, V., Burghoffer, B., Saliba, F., Mathieu, D., Samuel, D., Castaing, D., Petit, J.-C., Dussaix, E., Arlet, G. (2006). First outbreak of multidrug-resistant Klebsiella pneumoniae carrying blaVIM-1 and blaSHV-5 in a French university hospital. J Antimicrob Chemother 57: 142-145 [Abstract] [Full Text]  
• Basim, H., Minsavage, G. V., Stall, R. E., Wang, J.-F., Shanker, S., Jones, J. B. (2005). Characterization of a Unique Chromosomal Copper Resistance Gene Cluster from Xanthomonas campestris pv. vesicatoria. Appl. Environ. Microbiol. 71: 8284-8291 [Abstract] [Full Text]  
• Miko, A., Pries, K., Schroeter, A., Helmuth, R. (2005). Molecular mechanisms of resistance in multidrug-resistant serovars of Salmonella enterica isolated from foods in Germany. J Antimicrob Chemother 56: 1025-1033 [Abstract] [Full Text]  
• Robin, F., Delmas, J., Chanal, C., Sirot, D., Sirot, J., Bonnet, R. (2005). TEM-109 (CMT-5), a Natural Complex Mutant of TEM-1 {beta}-Lactamase Combining the Amino Acid Substitutions of TEM-6 and TEM-33 (IRT-5). Antimicrob. Agents Chemother. 49: 4443-4447 [Abstract] [Full Text]  
• Delmas, J., Robin, F., Bittar, F., Chanal, C., Bonnet, R. (2005). Unexpected Enzyme TEM-126: Role of Mutation Asp179Glu. Antimicrob. Agents Chemother. 49: 4280-4287 [Abstract] [Full Text]  
• Sekiguchi, J.-i., Asagi, T., Miyoshi-Akiyama, T., Fujino, T., Kobayashi, I., Morita, K., Kikuchi, Y., Kuratsuji, T., Kirikae, T. (2005). Multidrug-Resistant Pseudomonas aeruginosa Strain That Caused an Outbreak in a Neurosurgery Ward and Its aac(6')-Iae Gene Cassette Encoding a Novel Aminoglycoside Acetyltransferase. Antimicrob. Agents Chemother. 49: 3734-3742 [Abstract] [Full Text]  
• Ahmed, A. M, Kawamoto, H., Inouye, K., Hashiwata, Y., Sakaki, M., Seno, M., Shimamoto, T. (2005). Genomic analysis of a multidrug-resistant strain of enterohaemorrhagic Escherichia coli O157 : H7 causing a family outbreak in Japan. J Med Microbiol 54: 867-872 [Abstract] [Full Text]  
• Wei, Z.-Q., Chen, Y.-G., Yu, Y.-S., Lu, W.-X., Li, L.-J. (2005). Nosocomial spread of multi-resistant Klebsiella pneumoniae containing a plasmid encoding multiple {beta}-lactamases. J Med Microbiol 54: 885-888 [Abstract] [Full Text]  
• Huang, I-F., Chiu, C.-H., Wang, M.-H., Wu, C.-Y., Hsieh, K.-S., Chiou, C. C. (2005). Outbreak of Dysentery Associated with Ceftriaxone-Resistant Shigella sonnei: First Report of Plasmid-Mediated CMY-2-Type AmpC {beta}-Lactamase Resistance in S. sonnei. J. Clin. Microbiol. 43: 2608-2612 [Abstract] [Full Text]  
• Su, L.-H., Wu, T.-L., Chia, J.-H., Chu, C., Kuo, A.-J., Chiu, C.-H. (2005). Increasing ceftriaxone resistance in Salmonella isolates from a university hospital in Taiwan. J Antimicrob Chemother 55: 846-852 [Abstract] [Full Text]  
• Ansaruzzaman, M, Sultana, M., Talukder, K. A, Alam, K, Matsushita, S, Safa, A., Khajanchi, B. K, Dutta, D. K, Islam, Z., Albert, M J., Nair, G B., Sack, D. A (2005). Isolation and characterization of provisional serovar Shigella boydii E16553 from diarrhoeal patients in Bangladesh. J Med Microbiol 54: 477-480 [Abstract] [Full Text]  
• Zhao, Y., Ma, Z., Sundin, G. W. (2005). Comparative Genomic Analysis of the pPT23A Plasmid Family of Pseudomonas syringae. J. Bacteriol. 187: 2113-2126 [Abstract] [Full Text]  
• Woodford, N., Tierno, P. M. Jr., Young, K., Tysall, L., Palepou, M.-F. I., Ward, E., Painter, R. E., Suber, D. F., Shungu, D., Silver, L. L., Inglima, K., Kornblum, J., Livermore, D. M. (2004). Outbreak of Klebsiella pneumoniae Producing a New Carbapenem- Hydrolyzing Class A {beta}-Lactamase, KPC-3, in a New York Medical Center. Antimicrob. Agents Chemother. 48: 4793-4799 [Abstract] [Full Text]  
• Foster, G. C., McGhee, G. C., Jones, A. L., Sundin, G. W. (2004). Nucleotide Sequences, Genetic Organization, and Distribution of pEU30 and pEL60 from Erwinia amylovora. Appl. Environ. Microbiol. 70: 7539-7544 [Abstract] [Full Text]  
• Yan, J.-J., Wu, J.-J., Ko, W.-C., Tsai, S.-H., Chuang, C.-L., Wu, H.-M., Lu, Y.-J., Li, J.-D. (2004). Plasmid-mediated 16S rRNA methylases conferring high-level aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae isolates from two Taiwanese hospitals. J Antimicrob Chemother 54: 1007-1012 [Abstract] [Full Text]  
• Kruger, T., Szabo, D., Keddy, K. H., Deeley, K., Marsh, J. W., Hujer, A. M., Bonomo, R. A., Paterson, D. L. (2004). Infections with Nontyphoidal Salmonella Species Producing TEM-63 or a Novel TEM Enzyme, TEM-131, in South Africa. Antimicrob. Agents Chemother. 48: 4263-4270 [Abstract] [Full Text]  
• Decre, D., Burghoffer, B., Gautier, V., Petit, J.-C., Arlet, G. (2004). Outbreak of multi-resistant Klebsiella oxytoca involving strains with extended-spectrum {beta}-lactamases and strains with extended-spectrum activity of the chromosomal {beta}-lactamase. J Antimicrob Chemother 54: 881-888 [Abstract] [Full Text]  
• Leflon-Guibout, V., Jurand, C., Bonacorsi, S., Espinasse, F., Guelfi, M. C., Duportail, F., Heym, B., Bingen, E., Nicolas-Chanoine, M.-H. (2004). Emergence and Spread of Three Clonally Related Virulent Isolates of CTX-M-15-Producing Escherichia coli with Variable Resistance to Aminoglycosides and Tetracycline in a French Geriatric Hospital. Antimicrob. Agents Chemother. 48: 3736-3742 [Abstract] [Full Text]  
• Martinez-Urtaza, J., Lozano-Leon, A., DePaola, A., Ishibashi, M., Shimada, K., Nishibuchi, M., Liebana, E. (2004). Characterization of Pathogenic Vibrio parahaemolyticus Isolates from Clinical Sources in Spain and Comparison with Asian and North American Pandemic Isolates. J. Clin. Microbiol. 42: 4672-4678 [Abstract] [Full Text]  
• Libisch, B., Gacs, M., Csiszar, K., Muzslay, M., Rokusz, L., Fuzi, M. (2004). Isolation of an Integron-Borne blaVIM-4 Type Metallo-{beta}-Lactamase Gene from a Carbapenem-Resistant Pseudomonas aeruginosa Clinical Isolate in Hungary. Antimicrob. Agents Chemother. 48: 3576-3578 [Abstract] [Full Text]  
• Gray, J. T., Hungerford, L. L., Fedorka-Cray, P. J., Headrick, M. L. (2004). Extended-Spectrum-Cephalosporin Resistance in Salmonella enterica Isolates of Animal Origin. Antimicrob. Agents Chemother. 48: 3179-3181 [Abstract] [Full Text]  
• Hurst, M. R. H., Glare, T. R., Jackson, T. A. (2004). Cloning Serratia entomophila Antifeeding Genes--a Putative Defective Prophage Active against the Grass Grub Costelytra zealandica. J. Bacteriol. 186: 5116-5128 [Abstract] [Full Text]  
• Guerra, B., Junker, E., Helmuth, R. (2004). Incidence of the Recently Described Sulfonamide Resistance Gene sul3 among German Salmonella enterica Strains Isolated from Livestock and Food. Antimicrob. Agents Chemother. 48: 2712-2715 [Abstract] [Full Text]  
• Martinez-Urtaza, J., Liebana, E., Garcia-Migura, L., Perez-Pineiro, P., Saco, M. (2004). Characterization of Salmonella enterica Serovar Typhimurium from Marine Environments in Coastal Waters of Galicia (Spain). Appl. Environ. Microbiol. 70: 4030-4034 [Abstract] [Full Text]  
• Le, T. A. H., Lejay-Collin, M., Grimont, P. A. D., Hoang, T. L., Nguyen, T. V., Grimont, F., Scavizzi, M. R. (2004). Endemic, Epidemic Clone of Salmonella enterica Serovar Typhi Harboring a Single Multidrug-Resistant Plasmid in Vietnam between 1995 and 2002. J. Clin. Microbiol. 42: 3094-3099 [Abstract] [Full Text]  
• Wachino, J.-i., Doi, Y., Yamane, K., Shibata, N., Yagi, T., Kubota, T., Ito, H., Arakawa, Y. (2004). Nosocomial Spread of Ceftazidime-Resistant Klebsiella pneumoniae Strains Producing a Novel Class A {beta}-Lactamase, GES-3, in a Neonatal Intensive Care Unit in Japan. Antimicrob. Agents Chemother. 48: 1960-1967 [Abstract] [Full Text]  
• Yamane, K., Doi, Y., Yokoyama, K., Yagi, T., Kurokawa, H., Shibata, N., Shibayama, K., Kato, H., Arakawa, Y. (2004). Genetic Environments of the rmtA Gene in Pseudomonas aeruginosa Clinical Isolates. Antimicrob. Agents Chemother. 48: 2069-2074 [Abstract] [Full Text]  
• Pagani, L., Mantengoli, E., Migliavacca, R., Nucleo, E., Pollini, S., Spalla, M., Daturi, R., Romero, E., Rossolini, G. M. (2004). Multifocal Detection of Multidrug-Resistant Pseudomonas aeruginosa Producing the PER-1 Extended-Spectrum {beta}-Lactamase in Northern Italy. J. Clin. Microbiol. 42: 2523-2529 [Abstract] [Full Text]  
• Weller, S. A., Stead, D. E., Young, J. P. W. (2004). Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other {alpha}-Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat. Appl. Environ. Microbiol. 70: 2779-2785 [Abstract] [Full Text]  
• Eckert, C., Gautier, V., Saladin-Allard, M., Hidri, N., Verdet, C., Ould-Hocine, Z., Barnaud, G., Delisle, F., Rossier, A., Lambert, T., Philippon, A., Arlet, G. (2004). Dissemination of CTX-M-Type {beta}-Lactamases among Clinical Isolates of Enterobacteriaceae in Paris, France. Antimicrob. Agents Chemother. 48: 1249-1255 [Abstract] [Full Text]  
• Chang, L.-L., Chen, H.-F., Chang, C.-Y., Lee, T.-M., Wu, W.-J. (2004). Contribution of integrons, and SmeABC and SmeDEF efflux pumps to multidrug resistance in clinical isolates of Stenotrophomonas maltophilia. J Antimicrob Chemother 53: 518-521 [Abstract] [Full Text]  
• Doi, Y., Yokoyama, K., Yamane, K., Wachino, J.-i., Shibata, N., Yagi, T., Shibayama, K., Kato, H., Arakawa, Y. (2004). Plasmid-Mediated 16S rRNA Methylase in Serratia marcescens Conferring High-Level Resistance to Aminoglycosides. Antimicrob. Agents Chemother. 48: 491-496 [Abstract] [Full Text]  
• Walckenaer, E., Poirel, L., Leflon-Guibout, V., Nordmann, P., Nicolas-Chanoine, M.-H. (2004). Genetic and Biochemical Characterization of the Chromosomal Class A {beta}-Lactamases of Raoultella (formerly Klebsiella) planticola and Raoultella ornithinolytica. Antimicrob. Agents Chemother. 48: 305-312 [Abstract] [Full Text]  
• Broker, D., Arenskotter, M., Legatzki, A., Nies, D. H., Steinbuchel, A. (2004). Characterization of the 101-Kilobase-Pair Megaplasmid pKB1, Isolated from the Rubber-Degrading Bacterium Gordonia westfalica Kb1. J. Bacteriol. 186: 212-225 [Abstract] [Full Text]  
• Nagano, N., Shibata, N., Saitou, Y., Nagano, Y., Arakawa, Y. (2003). Nosocomial Outbreak of Infections by Proteus mirabilis That Produces Extended-Spectrum CTX-M-2 Type {beta}-Lactamase. J. Clin. Microbiol. 41: 5530-5536 [Abstract] [Full Text]  
• Miko, A., Pries, K., Schroeter, A., Helmuth, R. (2003). Multiple-Drug Resistance in D-Tartrate-Positive Salmonella enterica Serovar Paratyphi B Isolates from Poultry Is Mediated by Class 2 Integrons Inserted into the Bacterial Chromosome. Antimicrob. Agents Chemother. 47: 3640-3643 [Abstract] [Full Text]  
• Talukder, K. A., Islam, M. A., Khajanchi, B. K., Dutta, D. K., Islam, Z., Safa, A., Alam, K., Hossain, A., Nair, G. B., Sack, D. A. (2003). Temporal Shifts in the Dominance of Serotypes of Shigella dysenteriae from 1999 to 2002 in Dhaka, Bangladesh. J. Clin. Microbiol. 41: 5053-5058 [Abstract] [Full Text]  
• Wu, T.-L., Chia, J.-H., Su, L.-H., Kuo, A.-J., Chu, C., Chiu, C.-H. (2003). Dissemination of Extended-Spectrum {beta}-Lactamase-Producing Enterobacteriaceae in Pediatric Intensive Care Units. J. Clin. Microbiol. 41: 4836-4838 [Abstract] [Full Text]  
• Capilla, S., Goni, P., Rubio, M. C., Castillo, J., Millan, L., Cerda, P., Sahagun, J., Pitart, C., Beltran, A., Gomez-Lus, R. (2003). Epidemiological Study of Resistance to Nalidixic Acid and Other Antibiotics in Clinical Yersinia enterocolitica O:3 Isolates. J. Clin. Microbiol. 41: 4876-4878 [Abstract] [Full Text]  
• Strauch, E., Goelz, G., Knabner, D., Konietzny, A., Lanka, E., Appel, B. (2003). A cryptic plasmid of Yersinia enterocolitica encodes a conjugative transfer system related to the regions of CloDF13 Mob and IncX Pil. Microbiology 149: 2829-2845 [Abstract] [Full Text]  
• Wain, J., Diem Nga, L. T., Kidgell, C., James, K., Fortune, S., Song Diep, T., Ali, T., O Gaora, P., Parry, C., Parkhill, J., Farrar, J., White, N. J., Dougan, G. (2003). Molecular Analysis of incHI1 Antimicrobial Resistance Plasmids from Salmonella Serovar Typhi Strains Associated with Typhoid Fever. Antimicrob. Agents Chemother. 47: 2732-2739 [Abstract] [Full Text]  
• Furushita, M., Shiba, T., Maeda, T., Yahata, M., Kaneoka, A., Takahashi, Y., Torii, K., Hasegawa, T., Ohta, M. (2003). Similarity of Tetracycline Resistance Genes Isolated from Fish Farm Bacteria to Those from Clinical Isolates. Appl. Environ. Microbiol. 69: 5336-5342 [Abstract] [Full Text]  
• Kristiansen, M. A. M., Sandvang, D., Rasmussen, T. B. (2003). In Vivo Development of Quinolone Resistance in Salmonella enterica Serotype Typhimurium DT104. J. Clin. Microbiol. 41: 4462-4464 [Abstract] [Full Text]  
• Morris, D., O'Hare, C., Glennon, M., Maher, M., Corbett-Feeney, G., Cormican, M. (2003). Extended-Spectrum {beta}-Lactamases in Ireland, Including a Novel Enzyme, TEM-102. Antimicrob. Agents Chemother. 47: 2572-2578 [Abstract] [Full Text]  
• Beauchef-Havard, A., Arlet, G., Gautier, V., Labia, R., Grimont, P., Philippon, A. (2003). Molecular and Biochemical Characterization of a Novel Class A {beta}-Lactamase (HER-1) from Escherichia hermannii. Antimicrob. Agents Chemother. 47: 2669-2673 [Abstract] [Full Text]  
• Siu, L. K., Lu, P.-L., Chen, J.-Y., Lin, F. M., Chang, S.-C. (2003). High-Level Expression of AmpC {beta}-Lactamase Due to Insertion of Nucleotides between -10 and -35 Promoter Sequences in Escherichia coli Clinical Isolates: Cases Not Responsive to Extended-Spectrum-Cephalosporin Treatment. Antimicrob. Agents Chemother. 47: 2138-2144 [Abstract] [Full Text]  
• Preston, K. E., Graffunder, E. M., Evans, A. M., Venezia, R. A. (2003). Survey of Plasmid-Associated Genetic Markers in Enterobacteriaceae with Reduced Susceptibilities to Cephalosporins. Antimicrob. Agents Chemother. 47: 2179-2185 [Abstract] [Full Text]