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J Bacteriol. 1981 March; 145(3): 1421-1424

Curing of the 2 mu DNA plasmid from Saccharomyces cerevisiae.

A Toh-e and R B Wickner

ABSTRACT

The 2 mu DNA plasmid is often eliminated from yeast cells when they are transformed with the 2 mu DNA-LEU2-pMB9 composite plasmid pJDB219. Since pJDB219 is subsequently lost with high frequency, derivatives lacking all 2 mu DNA can be prepared from any strain.

J Bacteriol. 1981 March; 145(3): 1421-1424




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