This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Iyobe, S Articles by Mitsuhashi, S Search for Related Content PubMed PubMed Citation Articles by Iyobe, S Articles by Mitsuhashi, S

Tn2001, a transposon encoding chloramphenicol resistance in Pseudomonas aeruginosa.

ABSTRACT

We isolated a new transposon, Tn2001, from the group P-2 plasmid Rms159-1 in Pseudomonas aeruginosa. Tn2001-encoded chloramphenicol resistance did not result from the formation of chloramphenicol acetyltransferase. Tn2001 was transposable between temperate phages and conjugative and nonconjugative plasmids belonging to various incompatibility groups, including P-1, P-3, P-4, P-5, P-7, and P-8 in P. aeruginosa. Transposition occurred independently of the general recombination ability of the Pseudomonas host, and its frequency varied between 10(-1) and 10(-8), depending upon the donor and recipient replicons. Tn2001 transposition also occurred in a recombination-deficient strain of Escherichia coli. Agarose gel electrophoresis and electron microscopic observations revealed that Tn2001 could transpose to different sites in the RP4 replicon and that the transposed deoxyribonucleic acid fragment was 2.1 kilobases long.

This article has been cited by other articles:

• Nagano, N., Cordevant, C., Nagano, Y. (2008). Upper and lower urinary tract infection caused by Klebsiella pneumoniae serotype K2 and CTX-M-15 -lactamase-producing serotype K1: a case report and characterization of serum killing resistance. J Med Microbiol 57: 121-124 [Abstract] [Full Text]  
• Haruta, S., Yamaguchi, H., Yamamoto, E. T., Eriguchi, Y., Nukaga, M., O'Hara, K., Sawai, T. (2000). Functional Analysis of the Active Site of a Metallo-beta -Lactamase Proliferating in Japan. Antimicrob. Agents Chemother. 44: 2304-2309 [Abstract] [Full Text]