This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Boronat, A Articles by Aguilar, J Search for Related Content PubMed PubMed Citation Articles by Boronat, A Articles by Aguilar, J

Metabolism of L-fucose and L-rhamnose in Escherichia coli: differences in induction of propanediol oxidoreductase.

ABSTRACT

Escherichia coli is capable of growing on L-fucose or L-rhamnose as a sole source of carbon and energy. When grown under anaerobic conditions on either sugar, a nicotinamide adenine dinucleotide-linked L-lactaldehyde:propanediol oxidoreductase activity is induced. The functioning of this enzyme results in the regeneration of oxidized nicotinamide adenine dinucleotide. Conditions of induction of the enzyme activity were studied and were found to display different characteristics on each sugar. In the rhamnose-grown cells, the increase in enzyme activity detected under inducing conditions was accompanied by the synthesis of propanediol oxidoreductase, as measured by the appearance in the extracts of a protein that reacts with propanediol oxidoreductase antibodies. In contrast, in fucose-grown cells, the level of propanediol oxidoreductase as measured by enzyme antibody-reacting material was high under noninducing and inducing conditions. Thus, the increase in enzyme activity detected in going from noninducing to inducing conditions in fucose-grown cells did not depend on the appearance of the specific protein but on the activation of the propanediol oxidoreductase already present in the cells in an inactive form. The propanediol oxidoreductase of both homologous systems should consequently be regulated by different control mechanisms.

This article has been cited by other articles:

• Altaras, N. E., Cameron, D. C. (1999). Metabolic Engineering of a 1,2-Propanediol Pathway in Escherichia coli. Appl. Environ. Microbiol. 65: 1180-1185 [Abstract] [Full Text]  
• Pellicer, M. T., Fernandez, C., Badia, J., Aguilar, J., Lin, E. C. C., Baldoma, L. (1999). Cross-induction of glc and ace Operons of Escherichia coli Attributable to Pathway Intersection. CHARACTERIZATION OF THE glc PROMOTER. J. Biol. Chem. 274: 1745-1752 [Abstract] [Full Text]  
• Lu, Z., Cabiscol, E., Obradors, N., Tamarit, J., Ros, J., Aguilar, J., Lin, E. C. C. (1998). Evolution of an Escherichia coli Protein with Increased Resistance to Oxidative Stress. J. Biol. Chem. 273: 8308-8316 [Abstract] [Full Text]