This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mayberry, W R Search for Related Content PubMed PubMed Citation Articles by Mayberry, W R

Dihydroxy and monohydroxy fatty acids in Legionella pneumophila.

ABSTRACT

Five strains of Legionella pneumophila were examined for the presence of hydroxy fatty acid. The cellular distribution of the fatty acids was also determined, as was the variation of hydroxy acid production on five growth media. The strains tested all produced approximately 5 mol% of hydroxy fatty acid, most of which was found in the nonextractable, alkali-stable, acid-labile (wall-associated, amide-linked) fraction. Three major hydroxy acids were found, along with several minor components. The major hydroxy acids were analyzed by thin-layer chromatography, gas-liquid chromatography, mass spectrometry, and infrared spectrophotometry. These compounds were tentatively identified as 3-hydroxy-12-methyltridecanoate, 3-hydroxy-n-eicosanoate, and a novel dihydroxy acid, 2,3-dihydroxy-12-methyltridecanoate. The total amount of hydroxy acid produced, as well as the profile of the hydroxy acids, remained relatively unchanged with respect to strain and growth medium.

This article has been cited by other articles:

• Arigi, E., Singh, S., Kahlili, A. H, Winter, H. C, Goldstein, I. J, Levery, S. B (2007). Characterization of neutral and acidic glycosphingolipids from the lectin-producing mushroom, Polyporus squamosus. Glycobiology 17: 754-766 [Abstract] [Full Text]  
• Heise, N., Gutierrez, A. L.S., Mattos, K. A., Jones, C., Wait, R., Previato, J. O., Mendonca-Previato, L. (2002). Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms. Glycobiology 12: 409-420 [Abstract] [Full Text]