This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Montelone, B A Articles by Prakash, L Search for Related Content PubMed PubMed Citation Articles by Montelone, B A Articles by Prakash, L

Spontaneous mitotic recombination in mms8-1, an allele of the CDC9 gene of Saccharomyces cerevisiae.

ABSTRACT

The methyl methane sulfonate (MMS)-sensitive mutation mms8-1 increases the rate of spontaneous mitotic intragenic recombination at five heteroallelic loci on three chromosomes. Complementation, segregation, and mapping studies indicate that mms8-1 is allelic to cdc9, known to be defective in deoxyribonucleic acid ligase. Both mms8-1 and cdc9 mutants are lethal in combination with the recombination-defective mutant rad52-1. Genetic analysis of spontaneous red/white sectors in an ade2-1/ade2-1 ade5/+ mms8-1/mms8-1 strain shows nonreciprocal recombinational events involving long chromosome segments. We also observe greater than expected rates of simultaneous recombination at loci on different chromosomes in both wild-type and mms8-1 mutants.

This article has been cited by other articles:

• Vijayakumar, S., Chapados, B. R., Schmidt, K. H., Kolodner, R. D., Tainer, J. A., Tomkinson, A. E. (2007). The C-terminal domain of yeast PCNA is required for physical and functional interactions with Cdc9 DNA ligase. Nucleic Acids Res 35: 1624-1637 [Abstract] [Full Text]  
• Guzder, S. N., Sommers, C. H., Prakash, L., Prakash, S. (2006). Complex Formation with Damage Recognition Protein Rad14 Is Essential for Saccharomyces cerevisiae Rad1-Rad10 Nuclease To Perform Its Function in Nucleotide Excision Repair In Vivo. Mol. Cell. Biol. 26: 1135-1141 [Abstract] [Full Text]  
• Refsland, E. W., Livingston, D. M. (2005). Interactions Among DNA Ligase I, the Flap Endonuclease and Proliferating Cell Nuclear Antigen in the Expansion and Contraction of CAG Repeat Tracts in Yeast. Genetics 171: 923-934 [Abstract] [Full Text]  
• Kuzminov, A. (2001). DNA replication meets genetic exchange: Chromosomal damage and its repair by homologous recombination. Proc. Natl. Acad. Sci. USA 98: 8461-8468 [Abstract] [Full Text]  
• Donahue, S. L., Corner, B. E., Bordone, L., Campbell, C. (2001). Mitochondrial DNA ligase function in Saccharomyces cerevisiae. Nucleic Acids Res 29: 1582-1589 [Abstract] [Full Text]  
• Ireland, M. J., Reinke, S. S., Livingston, D. M. (2000). The Impact of Lagging Strand Replication Mutations on the Stability of CAG Repeat Tracts in Yeast. Genetics 155: 1657-1665 [Abstract] [Full Text]  
• Merrill, B. J., Holm, C. (1999). A Requirement for Recombinational Repair in Saccharomyces cerevisiae Is Caused by DNA Replication Defects of mec1 Mutants. Genetics 153: 595-605 [Abstract] [Full Text]  
• Merrill, B. J., Holm, C. (1998). The RAD52 Recombinational Repair Pathway is Essential in pol30 (PCNA) Mutants That Accumulate Small Single-Stranded DNA Fragments During DNA Synthesis. Genetics 148: 611-624 [Abstract] [Full Text]  
• Sommers, C. H., Miller, E. J., Dujon, B., Prakash, S., Prakash, L. (1995). Conditional Lethality of Null Mutations in RTH1 That Encodes the Yeast Counterpart of a Mammalian 5`- to 3`-Exonuclease Required for Lagging Strand DNA Synthesis in Reconstituted Systems. J. Biol. Chem. 270: 4193-4196 [Abstract] [Full Text]