This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Watson, R Articles by Visentin, L P Search for Related Content PubMed PubMed Citation Articles by Watson, R Articles by Visentin, L P

 Previous Article  |  Next Article 

J Bacteriol. 1981 August; 147(2): 569-577

Identification and characterization of Col plasmids from classical colicin E-producing strains.

ABSTRACT

A series of transformants have been derived which carry the Col plasmids from 11 E-colicin-producing strains isolated by Fredericq or Hamon. The ColE plasmids identified included four of type E1, five of type E2, and two of a type designated E4 by Horak (Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 233:58-63, 1975). Strain K317 was shown to carry a ColE7 plasmid and a 2.7-megadalton (Md) ColE2imm plasmid which confers immunity to colicin E2 but not the ability to produce colicin. Other plasmids identified in the colicinogenic isolates were a 3.4-Md ColN plasmid and a 1.3-Md Col plasmid of unknown type. The ColE1 plasmids all continued replicating in cultures treated with chloramphenicol. Strains carrying the ColE4 or ColE3-CA38 plasmid exhibited partial sensitivity to their own colicins and exhibited an unusual clearing-zone morphology when overlaid on stabs of colicin E2- or E7-producing strains. Except for ColE1-K53, ColE1-K47, ColE2-CA42, and ColE7-K317, all of the ColE plasmids were found to be about 4.3 Md in size. ColE2-CA42 and ColE7-K317 are both about 3.9 Md and are the only two plasmids of the E2, E3, E4, and E7 types that did not yield a 0.39-Md deoxyribonucleic acid fragment as an EcoRI digestion product. The comparisons of the ColE plasmids suggest several structural and functional relationships among them.

This article has been cited by other articles:

• Yagura, M., Nishio, S.-y., Kurozumi, H., Wang, C.-f., Itoh, T. (2006). Anatomy of the Replication Origin of Plasmid ColE2-P9. J. Bacteriol. 188: 999-1010 [Abstract] [Full Text]  
• Penfold, C. N., Healy, B., Housden, N. G., Boetzel, R., Vankemmelbeke, M., Moore, G. R., Kleanthous, C., James, R. (2004). Flexibility in the Receptor-Binding Domain of the Enzymatic Colicin E9 Is Required for Toxicity against Escherichia coli Cells. J. Bacteriol. 186: 4520-4527 [Abstract] [Full Text]