This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Buck, G A Articles by Groman, N B Search for Related Content PubMed PubMed Citation Articles by Buck, G A Articles by Groman, N B

Identification of deoxyribonucleic acid restriction fragments of beta-converting corynebacteriophages that carry the gene for diphtheria toxin.

ABSTRACT

Deoxyribonucleic acid fragments bearing the gene for diphtheria toxin have been identified in restriction enzyme digests of deoxyribonucleic acids from beta-converting and gamma-nonconverting corynebacteriophages. A combination of physical and genetic evidence has established that the Bam HI band C fragment of beta phage deoxyribonucleic acid, which carries the specific phage attachment site (Buck and Groman, J. Bacteriol. 148:131-142, 1981), also carries most, and probably all, of the gene for diphtheria toxin. A detailed restriction map of this tox-bearing Bam HI fragment has been developed, and the locations and orientation of the tox gene and the attP site within this fragment have been established.

This article has been cited by other articles:

• Kaczorek, M, Delpeyroux, F, Chenciner, N, Streeck, R., Murphy, JR, Boquet, P, Tiollais, P (1983). Nucleotide sequence and expression of the diphtheria tox228 gene in Escherichia coli. Science 221: 855-858 [Abstract]  
• Leong, D, Coleman, K., Murphy, JR (1983). Cloned fragment A of diphtheria toxin is expressed and secreted into the periplasmic space of Escherichia coli K12. Science 220: 515-517 [Abstract]