This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kolodrubetz, D Articles by Schleif, R Search for Related Content PubMed PubMed Citation Articles by Kolodrubetz, D Articles by Schleif, R

 Previous Article  |  Next Article 

J Bacteriol. 1981 November; 148(2): 472-479

L-arabinose transport systems in Escherichia coli K-12.

ABSTRACT

Mutations in the arabinose transport operons of Escherichia coli K-12 were isolated with the Mu lac phage by screening for cells in which beta-galactosidase is induced in the presence of L-arabinose. Standard genetic techniques were then used to isolate numerous mutations in either of the two transport systems. Complementation tests revealed only one gene, araE, in the low-affinity arabinose uptake system. P1 transduction placed araE between lysA (60.9 min) and thyA (60.5 min) and closer to lysA. The operon of the high-affinity transport system was found to contain two genes: araF, which codes for the arabinose-binding protein, and a new gene, araG. The newly identified gene, araG, was shown by two-dimensional gel electrophoresis to encode a protein which is located in the membrane. Only defects in araG could abolish uptake by the high-affinity system under the conditions we used.

This article has been cited by other articles:

• Khlebnikov, A., Risa, O., Skaug, T., Carrier, T. A., Keasling, J. D. (2000). Regulatable Arabinose-Inducible Gene Expression System with Consistent Control in All Cells of a Culture. J. Bacteriol. 182: 7029-7034 [Abstract] [Full Text]  
• Carolé, S., Pichoff, S., Bouché, J.-P. (1999). Escherichia coli Gene ydeA Encodes a Major Facilitator Pump Which Exports L-Arabinose and Isopropyl-beta -D-Thiogalactopyranoside. J. Bacteriol. 181: 5123-5125 [Abstract] [Full Text]  
• Bost, S., Silva, F., Belin, D. (1999). Transcriptional Activation of ydeA, Which Encodes a Member of the Major Facilitator Superfamily, Interferes with Arabinose Accumulation and Induction of the Escherichia coli Arabinose PBAD Promoter. J. Bacteriol. 181: 2185-2191 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]