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J Bacteriol. 1982 February; 149(2): 508-514

Partial purification and cleavage specificity of a site-specific endonuclease, SciNI, isolated from Spiroplasma citri.

M A Stephens

ABSTRACT

A site-specific endonuclease, SciNI, has been partially purified from the plant pathogen Spiroplasma citri. The enzyme recognizes the sequence 5'-G-C-G-C-3' and cleaves between the first G and C. 3'-C-G-C-G-5' SciNI is an isoschizomer of HhaI, but generates DNA fragments with 5' rather than 3' single-stranded protrusions.

J Bacteriol. 1982 February; 149(2): 508-514






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