This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Duncan, B K Articles by Weiss, B Search for Related Content PubMed PubMed Citation Articles by Duncan, B K Articles by Weiss, B

Specific mutator effects of ung (uracil-DNA glycosylase) mutations in Escherichia coli.

ABSTRACT

Studies of trpA reversions revealed that G:C leads to A:T transitions were stimulated about 30-fold in E. coli ung mutants, whereas other base substitutions were not affected. A dUTPase (dut) mutation, which increases the incorporation of uracil into DNA in place of thymine, had no significant effect on the rate of G:C leads to A:T transitions. The results support the proposal that the glycosylase functions to reduce the mutation rate in wild-type cells by acting in the repair of DNA cytosine residues that have undergone spontaneous deamination to uracil. Further support was provided by the finding that when lambda bacteriophages were treated with bisulfite, an agent known to produce cytosine deamination, the frequency of clear-plaque mutants was increased an additional 20-fold by growth on an ung host. Bisulfite-induced mutations of the cellular chromosome, however, were about equal in ung+ and ung strains; it was found that during the treatment of ung+ cells with bisulfite, the glycosylase was inactivated.

This article has been cited by other articles:

• Budke, B., Kuzminov, A. (2006). Hypoxanthine Incorporation Is Nonmutagenic in Escherichia coli.. J. Bacteriol. 188: 6553-6560 [Abstract] [Full Text]  
• Nagalingam, A., Seo, K.-Y., Loechler, E. L. (2005). Mutagenesis studies of the major benzo[a]pyrene N2-dG adduct in a 5'-TG versus a 5'-UG sequence: removal of the methyl group causes a modest decrease in the [G->T/G->A] mutational ratio. Mutagenesis 20: 105-110 [Abstract] [Full Text]  
• Ogasawara, H., Teramoto, J., Hirao, K., Yamamoto, K., Ishihama, A., Utsumi, R. (2004). Negative Regulation of DNA Repair Gene (ung) Expression by the CpxR/CpxA Two-Component System in Escherichia coli K-12 and Induction of Mutations by Increased Expression of CpxR. J. Bacteriol. 186: 8317-8325 [Abstract] [Full Text]  
• Guillet, M., Boiteux, S. (2003). Origin of Endogenous DNA Abasic Sites in Saccharomyces cerevisiae. Mol. Cell. Biol. 23: 8386-8394 [Abstract] [Full Text]  
• Postel, E. H., Abramczyk, B. M. (2003). Escherichia coli nucleoside diphosphate kinase is a uracil-processing DNA repair nuclease. Proc. Natl. Acad. Sci. USA 100: 13247-13252 [Abstract] [Full Text]  
• Acharya, N., Kumar, P., Varshney, U. (2003). Complexes of the uracil-DNA glycosylase inhibitor protein, Ugi, with Mycobacterium smegmatis and Mycobacterium tuberculosis uracil-DNA glycosylases. Microbiology 149: 1647-1658 [Abstract] [Full Text]  
• Venkatesh, J., Kumar, P., Krishna, P. S. M., Manjunath, R., Varshney, U. (2003). Importance of Uracil DNA Glycosylase in Pseudomonas aeruginosa and Mycobacterium smegmatis, G+C-rich Bacteria, in Mutation Prevention, Tolerance to Acidified Nitrite, and Endurance in Mouse Macrophages. J. Biol. Chem. 278: 24350-24358 [Abstract] [Full Text]  
• De Silva, F. S., Moss, B. (2002). Vaccinia Virus Uracil DNA Glycosylase Has an Essential Role in DNA Synthesis That Is Independent of Its Glycosylase Activity: Catalytic Site Mutations Reduce Virulence but Not Virus Replication in Cultured Cells. J. Virol. 77: 159-166 [Abstract] [Full Text]  
• Terato, H., Masaoka, A., Asagoshi, K., Honsho, A., Ohyama, Y., Suzuki, T., Yamada, M., Makino, K., Yamamoto, K., Ide, H. (2002). Novel repair activities of AlkA (3-methyladenine DNA glycosylase II) and endonuclease VIII for xanthine and oxanine, guanine lesions induced by nitric oxide and nitrous acid. Nucleic Acids Res 30: 4975-4984 [Abstract] [Full Text]  
• Kavli, B., Sundheim, O., Akbari, M., Otterlei, M., Nilsen, H., Skorpen, F., Aas, P. A., Hagen, L., Krokan, H. E., Slupphaug, G. (2002). hUNG2 Is the Major Repair Enzyme for Removal of Uracil from U:A Matches, U:G Mismatches, and U in Single-stranded DNA, with hSMUG1 as a Broad Specificity Backup. J. Biol. Chem. 277: 39926-39936 [Abstract] [Full Text]  
• Courcelle, C. T., Courcelle, J., Prichard, M. N., Mocarski, E. S. (2001). Requirement for Uracil-DNA Glycosylase during the Transition to Late-Phase Cytomegalovirus DNA Replication. J. Virol. 75: 7592-7601 [Abstract] [Full Text]  
• Nilsen, H., Krokan, H. E. (2001). Base excision repair in a network of defence and tolerance. Carcinogenesis 22: 987-998 [Full Text]  
• Nilsen, H., Steinsbekk, K. S., Otterlei, M., Slupphaug, G., Aas, P. A., Krokan, H. E. (2000). Analysis of uracil-DNA glycosylases from the murine Ung gene reveals differential expression in tissues and in embryonic development and a subcellular sorting pattern that differs from the human homologues. Nucleic Acids Res 28: 2277-2285 [Abstract] [Full Text]  
• Lutsenko, E., Bhagwat, A. S. (1999). The Role of the Escherichia coli Mug Protein in the Removal of Uracil and 3,N4-Ethenocytosine from DNA. J. Biol. Chem. 274: 31034-31038 [Abstract] [Full Text]  
• Guillouet, S., Rodal, A. A., An, G.-H., Lessard, P. A., Sinskey, A. J. (1999). Expression of the Escherichia coli Catabolic Threonine Dehydratase in Corynebacterium glutamicum and Its Effect on Isoleucine Production. Appl. Environ. Microbiol. 65: 3100-3107 [Abstract] [Full Text]  
• Fijalkowska, I. J., Jonczyk, P., Tkaczyk, M. M., Bialoskorska, M., Schaaper, R. M. (1998). Unequal fidelity of leading strand and lagging strand DNA replication on the Escherichia coli chromosome. Proc. Natl. Acad. Sci. USA 95: 10020-10025 [Abstract] [Full Text]  
• Beletskii, A., Bhagwat, A. S. (1996). Transcription-induced mutations: Increase in C to T mutations in the nontranscribed strand during transcription in Escherichia coli. Proc. Natl. Acad. Sci. USA 93: 13919-13924 [Abstract] [Full Text]  
• Barak, Y., Cohen-Fix, O., Livneh, Z. (1995). Deamination of Cytosine-containing Pyrimidine Photodimers in UV-irradiated DNA. J. Biol. Chem. 270: 24174-24179 [Abstract] [Full Text]