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J Bacteriol. 1982 November; 152(2): 567-571

Characterization of eight beta-lactamases of Gram-negative bacteria.

T Sawai, M Kanno and K Tsukamoto

ABSTRACT

Eight kinds of beta-lactamases produced by gram-negative bacteria were characterized by the following properties: molecular weight, isoelectric point, pH optimum, molecular activity, immunochemical reactivity, and kinetic parameters with respect to twelve kinds of common beta-lactam antibiotics. These beta-lactamases included two types of penicillinases mediated by R plasmids and six kinds of species-specific cephalosporinases. To determine a reliable value of the kinetic parameter, Km, we introduced a continuous and acidimetric assay method of beta-lactamase activity with a pH stat.

J Bacteriol. 1982 November; 152(2): 567-571




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