This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Reusch, V M Articles by Hurly, B J Search for Related Content PubMed PubMed Citation Articles by Reusch, V M, Jr Articles by Hurly, B J

 Previous Article  |  Next Article 

J Bacteriol. 1982 December; 152(3): 1147-1153

Levels of cell wall enzymes in endospores and vegetative cells of Bacillus subtilis.

ABSTRACT

Vegetative bacilli and refractile endospores of Bacillus subtilis 168 were disrupted by homogenization with glass beads and fractionated by differential centrifugation. Most of the protein of endospores was particulate, whereas for bacilli most was soluble. Alanine racemase activity was sixfold higher in extract of endospores than in extract of bacilli and was particulate, whereas the enzyme from bacilli was soluble. The specific activities of seven other enzymes involved in peptidoglycan and teichoic acid biosynthesis were higher in extracts of bacilli than in those of endospores. The results suggest that restoration of activities of these seven enzymes to vegetative levels occurs during germination and outgrowth.

This article has been cited by other articles:

• Bron, P. A., Benchimol, M. G., Lambert, J., Palumbo, E., Deghorain, M., Delcour, J., de Vos, W. M., Kleerebezem, M., Hols, P. (2002). Use of the alr Gene as a Food-Grade Selection Marker in Lactic Acid Bacteria. Appl. Environ. Microbiol. 68: 5663-5670 [Abstract] [Full Text]