This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Berger, H Articles by Goebel, W Search for Related Content PubMed PubMed Citation Articles by Berger, H Articles by Goebel, W

 Previous Article  |  Next Article 

J Bacteriol. 1982 December; 152(3): 1241-1247

Cloning of the chromosomal determinants encoding hemolysin production and mannose-resistant hemagglutination in Escherichia coli.

ABSTRACT

We have cloned the chromosomal hemolysin determinants from Escherichia coli strains belonging to the four O-serotypes O4, O6, O18, and O75. The hemolysin-producing clones were isolated from gene banks of these strains which were constructed by inserting partial Sau3A fragments of chromosomal DNA into the cosmid pJC74. The hemolytic cosmid clones were relatively stable. The inserts were further subcloned either as SalI fragments in pACYC184 or as BamHI-SalI fragments in a recombinant plasmid (pANN202) containing cistron C (hlyC) of the plasmid-encoded hemolysin determinant. Detailed restriction maps of each of these determinants were constructed, and it was found that, despite sharing overall homology, the determinants exhibited minor specific differences in their structure. These appeared to be restricted to cistron A (hlyA), which is the structural gene for hemolysin. In the gene banks of two of these hemolytic strains, we could also identify clones which carried the genetic determinants for the mannose-resistant hemagglutination antigens Vb and VIc. Both of these fimbrial antigens were expressed in the E. coli K-12 clones to an extent similar to that observed in the wild-type strains. These recombinant cosmids were rather unstable, and, in the absence of selection, segregated at a high frequency.

This article has been cited by other articles:

• Valle, J., Mabbett, A. N., Ulett, G. C., Toledo-Arana, A., Wecker, K., Totsika, M., Schembri, M. A., Ghigo, J.-M., Beloin, C. (2008). UpaG, a New Member of the Trimeric Autotransporter Family of Adhesins in Uropathogenic Escherichia coli. J. Bacteriol. 190: 4147-4161 [Abstract] [Full Text]  
• Zdziarski, J., Svanborg, C., Wullt, B., Hacker, J., Dobrindt, U. (2008). Molecular Basis of Commensalism in the Urinary Tract: Low Virulence or Virulence Attenuation?. Infect. Immun. 76: 695-703 [Abstract] [Full Text]  
• Rennemeier, C., Hammerschmidt, S., Niemann, S., Inamura, S., Zahringer, U., Kehrel, B. E. (2007). Thrombospondin-1 promotes cellular adherence of Gram-positive pathogens via recognition of peptidoglycan. FASEB J. 21: 3118-3132 [Abstract] [Full Text]  
• Engel, D., Dobrindt, U., Tittel, A., Peters, P., Maurer, J., Gutgemann, I., Kaissling, B., Kuziel, W., Jung, S., Kurts, C. (2006). Tumor Necrosis Factor Alpha- and Inducible Nitric Oxide Synthase-Producing Dendritic Cells Are Rapidly Recruited to the Bladder in Urinary Tract Infection but Are Dispensable for Bacterial Clearance. Infect. Immun. 74: 6100-6107 [Abstract] [Full Text]  
• Muller, C. M., Dobrindt, U., Nagy, G., Emody, L., Uhlin, B. E., Hacker, J. (2006). Role of Histone-Like Proteins H-NS and StpA in Expression of Virulence Determinants of Uropathogenic Escherichia coli.. J. Bacteriol. 188: 5428-5438 [Abstract] [Full Text]  
• Reisner, A., Krogfelt, K. A., Klein, B. M., Zechner, E. L., Molin, S. (2006). In Vitro Biofilm Formation of Commensal and Pathogenic Escherichia coli Strains: Impact of Environmental and Genetic Factors.. J. Bacteriol. 188: 3572-3581 [Abstract] [Full Text]  
• Holden, N. J., Totsika, M., Mahler, E., Roe, A. J., Catherwood, K., Lindner, K., Dobrindt, U., Gally, D. L. (2006). Demonstration of regulatory cross-talk between P fimbriae and type 1 fimbriae in uropathogenic Escherichia coli.. Microbiology 152: 1143-1153 [Abstract] [Full Text]  
• Sherlock, O., Dobrindt, U., Jensen, J. B., Munk Vejborg, R., Klemm, P. (2006). Glycosylation of the Self-Recognizing Escherichia coli Ag43 Autotransporter Protein.. J. Bacteriol. 188: 1798-1807 [Abstract] [Full Text]  
• Beloin, C., Michaelis, K., Lindner, K., Landini, P., Hacker, J., Ghigo, J.-M., Dobrindt, U. (2006). The Transcriptional Antiterminator RfaH Represses Biofilm Formation in Escherichia coli. J. Bacteriol. 188: 1316-1331 [Abstract] [Full Text]  
• Roos, V., Ulett, G. C., Schembri, M. A., Klemm, P. (2006). The Asymptomatic Bacteriuria Escherichia coli Strain 83972 Outcompetes Uropathogenic E. coli Strains in Human Urine. Infect. Immun. 74: 615-624 [Abstract] [Full Text]  
• Blumer, C., Kleefeld, A., Lehnen, D., Heintz, M., Dobrindt, U., Nagy, G., Michaelis, K., Emody, L., Polen, T., Rachel, R., Wendisch, V. F., Unden, G. (2005). Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli. Microbiology 151: 3287-3298 [Abstract] [Full Text]  
• Julia, O., Vives-Rego, J. (2005). Skew-Laplace distribution in Gram-negative bacterial axenic cultures: new insights into intrinsic cellular heterogeneity. Microbiology 151: 749-755 [Abstract] [Full Text]  
• Wehkamp, J., Harder, J., Wehkamp, K., Meissner, B. W.-v., Schlee, M., Enders, C., Sonnenborn, U., Nuding, S., Bengmark, S., Fellermann, K., Schroder, J. M., Stange, E. F. (2004). NF-{kappa}B- and AP-1-Mediated Induction of Human Beta Defensin-2 in Intestinal Epithelial Cells by Escherichia coli Nissle 1917: a Novel Effect of a Probiotic Bacterium. Infect. Immun. 72: 5750-5758 [Abstract] [Full Text]  
• Schneider, G., Dobrindt, U., Bruggemann, H., Nagy, G., Janke, B., Blum-Oehler, G., Buchrieser, C., Gottschalk, G., Emody, L., Hacker, J. (2004). The Pathogenicity Island-Associated K15 Capsule Determinant Exhibits a Novel Genetic Structure and Correlates with Virulence in Uropathogenic Escherichia coli Strain 536. Infect. Immun. 72: 5993-6001 [Abstract] [Full Text]  
• Grozdanov, L., Raasch, C., Schulze, J., Sonnenborn, U., Gottschalk, G., Hacker, J., Dobrindt, U. (2004). Analysis of the Genome Structure of the Nonpathogenic Probiotic Escherichia coli Strain Nissle 1917. J. Bacteriol. 186: 5432-5441 [Abstract] [Full Text]  
• Middendorf, B., Hochhut, B., Leipold, K., Dobrindt, U., Blum-Oehler, G., Hacker, J. (2004). Instability of Pathogenicity Islands in Uropathogenic Escherichia coli 536. J. Bacteriol. 186: 3086-3096 [Abstract] [Full Text]  
• Grozdanov, L., Zahringer, U., Blum-Oehler, G., Brade, L., Henne, A., Knirel, Y. A., Schombel, U., Schulze, J., Sonnenborn, U., Gottschalk, G., Hacker, J., Rietschel, E. T., Dobrindt, U. (2002). A Single Nucleotide Exchange in the wzy Gene Is Responsible for the Semirough O6 Lipopolysaccharide Phenotype and Serum Sensitivity of Escherichia coli Strain Nissle 1917. J. Bacteriol. 184: 5912-5925 [Abstract] [Full Text]  
• Nagy, G., Dobrindt, U., Schneider, G., Khan, A. S., Hacker, J., Emody, L. (2002). Loss of Regulatory Protein RfaH Attenuates Virulence of Uropathogenic Escherichia coli. Infect. Immun. 70: 4406-4413 [Abstract] [Full Text]  
• Nagy, G., Dobrindt, U., Kupfer, M., Emody, L., Karch, H., Hacker, J. (2001). Expression of Hemin Receptor Molecule ChuA Is Influenced by RfaH in Uropathogenic Escherichia coli Strain 536. Infect. Immun. 69: 1924-1928 [Abstract] [Full Text]