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J Bacteriol. 1983 February; 153(2): 610-615

Origin of Escherichia coli K-12 Hfr B7.

M J Virolle, J P Gélugne, S Béjar and J P Bouché

ABSTRACT

Several F' plasmids encoding resistance to tetracycline have been derived from a trg::Tn10 Hfr B7 strain of Escherichia coli K-12. One of these plasmids, JGF312, was analyzed by restriction endonuclease digestion and Southern blot hybridization to cloned chromosomal fragments. This analysis revealed that JGF312 was formed by Tn10-promoted deletion from the Tn10 insertion (31.4 min) to within the prophage rac at 30.1 min. Hfr B7 was shown to result from recombination between IS2 of F delta (33-43) and a chromosomal IS2 located within the rac-man region at 30.9 min on the genetic map.

J Bacteriol. 1983 February; 153(2): 610-615






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