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J Bacteriol. 1983 April; 154(1): 177-184

Chromosomal location of the gene encoding phosphoribosylpyrophosphate synthetase in Escherichia coli.

B Hove-Jensen

ABSTRACT

A mutant of Escherichia coli with a partially defective phosphoribosylpyrophosphate synthetase (ribosephosphate pyrophosphokinase) has been characterized genetically. The genetic lesion causing the altered phosphoribosylpyrophosphate synthetase, prs, was mapped at 26 min on the linkage map by conjugation. Transductional analysis of the prs region established the gene order as purB-fadR-dadR-tre-pth-prs-hemA-trp. Two additional mutations were identified in the mutant: one in gsk, the gene encoding guanosine kinase, and one in lon, conferring a mucoid colony morphology. The contribution of each mutation to the phenotype of the mutant has been evaluated.

J Bacteriol. 1983 April; 154(1): 177-184




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