This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dickson, R C Articles by Barr, K Search for Related Content PubMed PubMed Citation Articles by Dickson, R C Articles by Barr, K

 Previous Article  |  Next Article 

J Bacteriol. 1983 June; 154(3): 1245-1251

Characterization of lactose transport in Kluyveromyces lactis.

ABSTRACT

We have determined that lactose uptake in Kluyveromyces lactis is mediated by an inducible transport system. Induction, elicited by lactose or galactose, of the transporter required protein synthesis. Transport of lactose required an energy-generating system and occurred by an active process, since an intracellular lactose concentration 175 times greater than the extracellular concentration could be obtained. The Km for lactose transport was about 2.8 mM in uninduced and lactose- or galactose-induced cells. The lactose transporters in K. lactis and Escherichia coli appear to be different since they respond uniquely to inhibition by substrate analogs.

This article has been cited by other articles:

• Guimaraes, P. M. R., Multanen, J.-P., Domingues, L., Teixeira, J. A., Londesborough, J. (2008). Stimulation of Zero-trans Rates of Lactose and Maltose Uptake into Yeasts by Preincubation with Hexose To Increase the Adenylate Energy Charge. Appl. Environ. Microbiol. 74: 3076-3084 [Abstract] [Full Text]  
• Anders, A., Lilie, H., Franke, K., Kapp, L., Stelling, J., Gilles, E. D., Breunig, K. D. (2006). The Galactose Switch in Kluyveromyces lactis Depends on Nuclear Competition between Gal4 and Gal1 for Gal80 Binding. J. Biol. Chem. 281: 29337-29348 [Abstract] [Full Text]  
• Wiedemuth, C., Breunig, K. D. (2005). Role of Snf1p in Regulation of Intracellular Sorting of the Lactose and Galactose Transporter Lac12p in Kluyveromyces lactis. Eukaryot Cell 4: 716-721 [Abstract] [Full Text]  
• Seiboth, B., Hartl, L., Salovuori, N., Lanthaler, K., Robson, G. D., Vehmaanpera, J., Penttila, M. E., Kubicek, C. P. (2005). Role of the bga1-Encoded Extracellular {beta}-Galactosidase of Hypocrea jecorina in Cellulase Induction by Lactose. Appl. Environ. Microbiol. 71: 851-857 [Abstract] [Full Text]