This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yonekawa, H Articles by Parkinson, J S Search for Related Content PubMed PubMed Citation Articles by Yonekawa, H Articles by Parkinson, J S

 Previous Article  |  Next Article 

J Bacteriol. 1983 December; 156(3): 1228-1235

Requirement of the cheB function for sensory adaptation in Escherichia coli.

ABSTRACT

The chemotactic behavior of Escherichia coli mutants defective in cheB function, which is required to remove methyl esters from methyl-accepting chemotaxis proteins, was investigated by subjecting swimming or antibody-tethered cells to various attractant chemicals. Two cheB point mutants, one missense and one nonsense, exhibited stimulus response times much longer than did the wild type, but they eventually returned to the prestimulus swimming pattern, indicating that they were not completely defective in sensory adaptation. In contrast, strains deleted for the cheB function showed no evidence of adaptation ability after stimulation. The crucial difference between these strains appeared to be the residual level of cheB-dependent methylesterase activity they contained. Both point mutants showed detectable levels of methanol evolution due to turnover of methyl groups on methyl-accepting chemotaxis protein molecules, whereas the cheB deletion mutant did not. In addition, it was possible to incorporate the methyl label into the methyl-accepting chemotaxis proteins of the point mutants but not into those of the cheB deletion strain. These findings indicate that cheB function is essential for sensory adaptation in Escherichia coli.

This article has been cited by other articles:

• Kim, T.-J., Young, B. M., Young, G. M. (2008). Effect of Flagellar Mutations on Yersinia enterocolitica Biofilm Formation. Appl. Environ. Microbiol. 74: 5466-5474 [Abstract] [Full Text]  
• Stephens, B. B., Loar, S. N., Alexandre, G. (2006). Role of CheB and CheR in the Complex Chemotactic and Aerotactic Pathway of Azospirillum brasilense. J. Bacteriol. 188: 4759-4768 [Abstract] [Full Text]  
• Bibikov, S. I., Miller, A. C., Gosink, K. K., Parkinson, J. S. (2004). Methylation-Independent Aerotaxis Mediated by the Escherichia coli Aer Protein. J. Bacteriol. 186: 3730-3737 [Abstract] [Full Text]  
• Lamanna, A. C., Gestwicki, J. E., Strong, L. E., Borchardt, S. L., Owen, R. M., Kiessling, L. L. (2002). Conserved Amplification of Chemotactic Responses through Chemoreceptor Interactions. J. Bacteriol. 184: 4981-4987 [Abstract] [Full Text]  
• Martin, A. C., Wadhams, G. H., Shah, D. S. H., Porter, S. L., Mantotta, J. C., Craig, T. J., Verdult, P. H., Jones, H., Armitage, J. P. (2001). CheR- and CheB-Dependent Chemosensory Adaptation System of Rhodobacter sphaeroides. J. Bacteriol. 183: 7135-7144 [Abstract] [Full Text]  
• Karatan, E., Saulmon, M. M., Bunn, M. W., Ordal, G. W. (2001). Phosphorylation of the Response Regulator CheV Is Required for Adaptation to Attractants during Bacillus subtilis Chemotaxis. J. Biol. Chem. 276: 43618-43626 [Abstract] [Full Text]  
• Maki, N., Gestwicki, J. E., Lake, E. M., Kiessling, L. L., Adler, J. (2000). Motility and Chemotaxis of Filamentous Cells of Escherichia coli. J. Bacteriol. 182: 4337-4342 [Abstract] [Full Text]