This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Surin, B P Articles by Cox, G B Search for Related Content PubMed PubMed Citation Articles by Surin, B P Articles by Cox, G B

 Previous Article  |  Next Article 

J Bacteriol. 1985 January; 161(1): 189-198

Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships.

ABSTRACT

The DNA nucleotide sequence of four genes for the phosphate-specific transport system of Escherichia coli is reported. Along with the DNA sequence for the phoS gene reported previously (Surin et al., J. Bacteriol. 157:772-778, 1984; Magota et al., J. Bacteriol. 157:909-917, 1984), this study completes the nucleotide sequence of the phosphate-specific transport region. The complete sequence (including phoS) contains five open reading frames oriented in the same direction, each preceded by a putative ribosome-binding site near the presumed translation initiation codon ATG. The complete sequence is transcribed counterclockwise, in the order phoS pstC pstA pstB phoU. Genetic complementation shows that of the four open reading frames in the new sequence, three correspond to known mutant alleles; the fourth, which was designated pstC, has not been described before and could not be related to any known mutant allele. We have confirmed that pstA was allelic to phoT32. The pstC, pstB, and phoU gene products were identified as peripheral membrane proteins. The pstA gene product appears to be an integral membrane protein.

This article has been cited by other articles:

• Bellenger, J. P., Wichard, T., Kraepiel, A. M. L. (2008). Vanadium Requirements and Uptake Kinetics in the Dinitrogen-Fixing Bacterium Azotobacter vinelandii. Appl. Environ. Microbiol. 74: 1478-1484 [Abstract] [Full Text]  
• Lubelski, J., Konings, W. N., Driessen, A. J. M. (2007). Distribution and Physiology of ABC-Type Transporters Contributing to Multidrug Resistance in Bacteria. Microbiol. Mol. Biol. Rev. 71: 463-476 [Abstract] [Full Text]  
• Rizk, S. S., Cuneo, M. J., Hellinga, H. W. (2006). Identification of cognate ligands for the Escherichia coli phnD protein product and engineering of a reagentless fluorescent biosensor for phosphonates. Protein Sci. 15: 1745-1751 [Abstract] [Full Text]  
• Buckles, E. L., Wang, X., Lockatell, C. V., Johnson, D. E., Donnenberg, M. S. (2006). PhoU enhances the ability of extraintestinal pathogenic Escherichia coli strain CFT073 to colonize the murine urinary tract. Microbiology 152: 153-160 [Abstract] [Full Text]  
• Lamarche, M. G., Dozois, C. M., Daigle, F., Caza, M., Curtiss, R. III, Dubreuil, J. D., Harel, J. (2005). Inactivation of the Pst System Reduces the Virulence of an Avian Pathogenic Escherichia coli O78 Strain. Infect. Immun. 73: 4138-4145 [Abstract] [Full Text]  
• Liu, J., Lou, Y., Yokota, H., Adams, P. D., Kim, R., Kim, S.-H. (2005). Crystal Structure of a PhoU Protein Homologue: A NEW CLASS OF METALLOPROTEIN CONTAINING MULTINUCLEAR IRON CLUSTERS. J. Biol. Chem. 280: 15960-15966 [Abstract] [Full Text]  
• Runyen-Janecky, L. J., Boyle, A. M., Kizzee, A., Liefer, L., Payne, S. M. (2005). Role of the Pst System in Plaque Formation by the Intracellular Pathogen Shigella flexneri. Infect. Immun. 73: 1404-1410 [Abstract] [Full Text]  
• Peirs, P., Lefevre, P., Boarbi, S., Wang, X.-M., Denis, O., Braibant, M., Pethe, K., Locht, C., Huygen, K., Content, J. (2005). Mycobacterium tuberculosis with Disruption in Genes Encoding the Phosphate Binding Proteins PstS1 and PstS2 Is Deficient in Phosphate Uptake and Demonstrates Reduced In Vivo Virulence. Infect. Immun. 73: 1898-1902 [Abstract] [Full Text]  
• White, A. K., Metcalf, W. W. (2004). The htx and ptx Operons of Pseudomonas stutzeri WM88 Are New Members of the Pho Regulon. J. Bacteriol. 186: 5876-5882 [Abstract] [Full Text]  
• Matsuzaki, M., Abe, M., Hara, S., Iwasaki, Y., Yamamoto, I., Satoh, T. (2003). An Abundant Periplasmic Protein of the Denitrifying Phototroph Rhodobacter sphaeroides f. sp. denitrificans is PstS, a Component of an ABC Phosphate Transport System. Plant Cell Physiol 44: 212-216 [Abstract] [Full Text]  
• Runyen-Janecky, L. J., Payne, S. M. (2002). Identification of Chromosomal Shigella flexneri Genes Induced by the Eukaryotic Intracellular Environment. Infect. Immun. 70: 4379-4388 [Abstract] [Full Text]  
• Hoffer, S. M., Tommassen, J. (2001). The Phosphate-Binding Protein of Escherichia coli Is Not Essential for Pi-Regulated Expression of the pho Regulon. J. Bacteriol. 183: 5768-5771 [Abstract] [Full Text]  
• Harris, R. M., Webb, D. C., Howitt, S. M., Cox, G. B. (2001). Characterization of PitA and PitB from Escherichia coli. J. Bacteriol. 183: 5008-5014 [Abstract] [Full Text]  
• Hoffer, S. M., Westerhoff, H. V., Hellingwerf, K. J., Postma, P. W., Tommassen, J. (2001). Autoamplification of a Two-Component Regulatory System Results in ""Learning"" Behavior. J. Bacteriol. 183: 4914-4917 [Abstract] [Full Text]  
• Hoffer, S. M., Schoondermark, P., van Veen, H. W., Tommassen, J. (2001). Activation by Gene Amplification of pitB, Encoding a Third Phosphate Transporter of Escherichia coli K-12. J. Bacteriol. 183: 4659-4663 [Abstract] [Full Text]  
• Ruiz-Lozano, J. M., Bonfante, P. (1999). Identification of a Putative P-Transporter Operon in the Genome of a Burkholderia Strain Living inside the Arbuscular Mycorrhizal Fungus Gigaspora margarita. J. Bacteriol. 181: 4106-4109 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
• Mitsukawa, N., Okumura, S., Shirano, Y., Sato, S., Kato, T., Harashima, S., Shibata, D. (1997). Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions. Proc. Natl. Acad. Sci. USA 94: 7098-7102 [Abstract] [Full Text]  
• Monaco, J., Cho, S, Attaya, M (1990). Transport protein genes in the murine MHC: possible implications for antigen processing. Science 250: 1723-1726 [Abstract]  
• Riordan, J., Rommens, J., Kerem, B, Alon, N, Rozmahel, R, Grzelczak, Z, Zielenski, J, Lok, S, Plavsic, N, Chou, J., al., e. (1989). Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073 [Abstract]  
• Sarin, J., Aggarwal, S., Chaba, R., Varshney, G. C., Chakraborti, P. K. (2001). B-subunit of Phosphate-specific Transporter from Mycobacterium tuberculosis Is a Thermostable ATPase. J. Biol. Chem. 276: 44590-44597 [Abstract] [Full Text]  
• Gatti, D., Mitra, B., Rosen, B. P. (2000). Escherichia coli Soft Metal Ion-translocating ATPases. J. Biol. Chem. 275: 34009-34012 [Full Text]