This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Abraham, L J Articles by Rood, J I Search for Related Content PubMed PubMed Citation Articles by Abraham, L J Articles by Rood, J I

Molecular analysis of transferable tetracycline resistance plasmids from Clostridium perfringens.

ABSTRACT

Conjugative tetracycline resistance plasmids from 15 Clostridium perfringens isolates from piggeries were analyzed by restriction endonuclease digestion and agarose gel electrophoresis. Seven isolates from one farm were found to carry a 47-kilobase pair (kb) plasmid, pJIR5, which had EcoRI, XbaI, and ClaI profiles that were identical to those of a previously characterized plasmid, pCW3. An isolate from a second farm was found to carry a plasmid, pJIR6, which also was indistinguishable from pCW3. Five additional isolates from a third farm carried a 67-kb plasmid, pJIR2, which had at least 29 kb of DNA in common with pCW3. Finally, two isolates from a fourth farm were found to carry a 50-kb plasmid pJIR4, which appeared to consist of an entire pCW3 molecule with a 3-kb insertion. Comparative restriction maps of pCW3, pJIR2, and pJIR4 that identified the regions of homology among these plasmids were constructed. We suggest that many conjugative tetracycline resistance plasmids in C. perfringens may contain a pCW3-like core.

This article has been cited by other articles:

• Parsons, J. A., Bannam, T. L., Devenish, R. J., Rood, J. I. (2007). TcpA, an FtsK/SpoIIIE Homolog, Is Essential for Transfer of the Conjugative Plasmid pCW3 in Clostridium perfringens. J. Bacteriol. 189: 7782-7790 [Abstract] [Full Text]  
• Hughes, M. L., Poon, R., Adams, V., Sayeed, S., Saputo, J., Uzal, F. A., McClane, B. A., Rood, J. I. (2007). Epsilon-Toxin Plasmids of Clostridium perfringens Type D Are Conjugative. J. Bacteriol. 189: 7531-7538 [Abstract] [Full Text]  
• Johanesen, P. A., Lyras, D., Bannam, T. L., Rood, J. I. (2001). Transcriptional Analysis of the tet(P) Operon from Clostridium perfringens. J. Bacteriol. 183: 7110-7119 [Abstract] [Full Text]  
• Awad, M. M., Ellemor, D. M., Boyd, R. L., Emmins, J. J., Rood, J. I. (2001). Synergistic Effects of Alpha-Toxin and Perfringolysin O in Clostridium perfringens-Mediated Gas Gangrene. Infect. Immun. 69: 7904-7910 [Abstract] [Full Text]  
• Farrow, K. A., Lyras, D., Rood, J. I. (2001). Genomic analysis of the erythromycin resistance element Tn5398 from Clostridium difficile. Microbiology 147: 2717-2728 [Abstract] [Full Text]  
• Brynestad, S., Sarker, M. R., McClane, B. A., Granum, P. E., Rood, J. I. (2001). Enterotoxin Plasmid from Clostridium perfringens Is Conjugative. Infect. Immun. 69: 3483-3487 [Abstract] [Full Text]  
• Farrow, K. A., Lyras, D., Rood, J. I. (2000). The Macrolide-Lincosamide-Streptogramin B Resistance Determinant from Clostridium difficile 630 Contains Two erm(B) Genes. Antimicrob. Agents Chemother. 44: 411-413 [Abstract] [Full Text]  
• Johnson, S., Samore, M. H., Farrow, K. A., Killgore, G. E., Tenover, F. C., Lyras, D., Rood, J. I., DeGirolami, P., Baltch, A. L., Rafferty, M. E., Pear, S. M., Gerding, D. N. (1999). Epidemics of Diarrhea Caused by a Clindamycin-Resistant Strain of Clostridium difficile in Four Hospitals. NEJM 341: 1645-1651 [Abstract] [Full Text]